Rapidly dividing Ehrlich ascites cells have a higher rate of protein synthesis than slowly-growing cells. This is true whether protein synthesis is measured in vizm or in a cell-free amino acid incorporating system. A lack of endogenous messenger RNA or a ribosomal defect in stationary cells is suggested as a likely cause of the decreased rate of protein synthesis.The soluble proteins synthesized by rapidly-dividing cells are qualitatively different from those synthesized by slowly-growing cells. The latter also have a larger intracellular pool of several amino acids and a slower protein turnover than rapidly-dividing cells.Ehrlich ascites tumors have been extensively investigated from a kinetic point of view, as models for chemotherapy or X-ray radiation and a cell suspension for basic biological studies. The hypotetraploid subline that has been studied for several years in one of our laboratories (Baserga. 19631, when inoculated intraperitoneally into mice, has two distinct phases: at the beginning, it grows exponentially, but after the seventh to eighth day (depending on the size of the inoculum and the strain and sex of mice), its growth declines until the number of tumor cells becomes stationary (Baserga, 1964).In an attempt to learn some of the factors that may control the proliferation of Ehrlich ascites cells, we have compared protein synthesis in exponentially growing cells with that of cells in the stationary phase.
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