In response to drought stress the phytohormone ABA (abscisic acid) induces stomatal closure and, therein, activates guard cell anion channels in a calcium-dependent as well as-independent manner. Two key components of the ABA signaling pathway are the protein kinase OST1 (open stomata 1) and the protein phosphatase ABI1 (ABA insensitive 1). The recently identified guard cell anion channel SLAC1 appeared to be the key ion channel in this signaling pathway but remained electrically silent when expressed heterologously. Using split YFP assays, we identified OST1 as an interaction partner of SLAC1 and ABI1. Upon coexpression of SLAC1 with OST1 in Xenopus oocytes, SLAC1-related anion currents appeared similar to those observed in guard cells. Integration of ABI1 into the SLAC1/OST1 complex, however, prevented SLAC1 activation. Our studies demonstrate that SLAC1 represents the slow, deactivating, weak voltage-dependent anion channel of guard cells controlled by phosphorylation/dephosphorylation.ABA signaling ͉ S-type anion channel ͉ OST1/ABI1

In response to drought stress, the phytohormone abscisic acid (ABA) induces stomatal closure. Thereby the stress hormone activates guard cell anion channels in a calcium-dependent, as well as -independent, manner. Open stomata 1 protein kinase (OST1) and ABI1 protein phosphatase (ABA insensitive 1) represent key components of calcium-independent ABA signaling. Recently, the guard cell anion channel SLAC1 was identified. When expressed heterologously SLAC1 remained electrically silent. Upon coexpression with Ca 2+ -independent OST1, however, SLAC1 anion channels appear activated in an ABI1-dependent manner. Mutants lacking distinct calcium-dependent protein kinases (CPKs) appeared impaired in ABA stimulation of guard cell ion channels, too. To study SLAC1 activation via the calcium-dependent ABA pathway, we studied the SLAC1 response to CPKs in the Xenopus laevis oocyte system. Split YFP-based protein-protein interaction assays, using SLAC1 as the bait, identified guard cell expressed CPK21 and 23 as major interacting partners. Upon coexpression of SLAC1 with CPK21 and 23, anion currents document SLAC1 stimulation by these guard cell protein kinases. Ca 2+ -sensitive activation of SLAC1, however, could be assigned to the CPK21 pathway only because CPK23 turned out to be rather Ca 2+ -insensitive. In line with activation by OST1, CPK activation of the guard cell anion channel was suppressed by ABI1. Thus the CPK and OST1 branch of ABA signal transduction in guard cells seem to converge on the level of SLAC1 under the control of the ABI1/ABA-receptor complex.abscisic acid signaling | drought stress | guard cell | S-type anion channel T he drought hormone abscisic acid (ABA) triggers release of K + and anions from guard cells and thereby causes stomatal closure (1, 2). Recently, SLAC1, a guard cell anion channel, was identified (3-5). In guard cells of these ABA-and CO 2 /O 3 -insensitive mutant plants, anion currents appeared largely suppressed. When SLAC1 was expressed with the open stomata 1 protein kinase (OST1) in Xenopus oocytes, SLAC1-related anion currents, similar to those observed in guard cells, appeared (6). The presence of ABI1, however, prevented SLAC1 activation. This ABA pathway resembles the Ca 2+ -independent activation of SLAC-type anion currents in guard cells. ABA signal transduction, however, has been shown to activate guard cell anion channels in a calcium-independent as well as -dependent manner (7-10). This became evident in abi1-1 mutant plants, where anion channels do not respond to ABA anymore (11) but still activate with calcium (12). Furthermore the described mutant growth controlled by abscisic acid (gca2) (13-14), isolated from the Arabidopsis ecotype Landsberg erecta, was shown to be impaired in ABA-induced stomatal closure in a Ca 2+ -dependent manner. Moreover [Ca 2+ ] cyt elevation was shown to result in activation of S-type anion channels via phosphorylation (12, 15), suggesting a role of phosphorylation events in [Ca 2+ ] cyt signaling.CDPKs resemble Ca 2+ -dependent Ser/Thr pr...

S-type anion channels are direct targets of abscisic acid (ABA) signaling and contribute to chloride and nitrate release from guard cells, which in turn initiates stomatal closure. SLAC1 was the first component of the guard cell S-type anion channel identified. However, we found that guard cells of Arabidopsis SLAC1 mutants exhibited nitrate conductance. SLAH3 (SLAC1 homolog 3) was also present in guard cells, and coexpression of SLAH3 with the calcium ion (Ca2+)-dependent kinase CPK21 in Xenopus oocytes mediated nitrate-induced anion currents. Nitrate, calcium, and phosphorylation regulated SLAH3 activity. CPK21-dependent SLAH3 phosphorylation and activation were blocked by ABI1, a PP2C-type protein phosphatase that is inhibited by ABA and inhibits the ABA signaling pathway in guard cells. We reconstituted the ABA-stimulated phosphorylation of the SLAH3 amino-terminal domain by CPK21 in vitro by including the ABA receptor-phosphatase complex RCAR1-ABI1 in the reactions. We propose that ABA perception by the complex consisting of ABA receptors of the RCAR/PYR/PYL family and ABI1 releases CPK21 from inhibition by ABI1, and then CPK21 is further activated by an increase in the cytosolic Ca2+ concentration, leading to its phosphorylation of SLAH3. Thus, the identification of SLAH3 as the nitrate-, calcium-, and ABA-sensitive guard cell anion channel provides insights into the relationship among stomatal response to drought, signaling by nitrate, and nitrate metabolism.

Stomata are pores on the leaf surface, bounded by two guard cells, which control the uptake of CO(2) for photosynthesis and the concomitant loss of water vapor. In 1898, Francis Darwin showed that stomata close in response to reduced atmospheric relative humidity (rh); however, our understanding of the signaling pathway responsible for coupling changes in rh to alterations in stomatal aperture is fragmentary. The results presented here highlight the primacy of abscisic acid (ABA) in the stomatal response to drying air. We show that guard cells possess the entire ABA biosynthesis pathway and that it appears upregulated by positive feedback by ABA. When wild-type Arabidopsis and the ABA-deficient mutant aba3-1 were exposed to reductions in rh, the aba3-1 mutant wilted, whereas the wild-type did not. However, when aba3-1 plants, in which ABA synthesis had been specifically rescued in guard cells, were challenged with dry air, they did not wilt. These data indicate that guard cell-autonomous ABA synthesis is required for and is sufficient for stomatal closure in response to low rh. Guard cell-autonomous ABA synthesis allows the plant to tailor leaf gas exchange exquisitely to suit the prevailing environmental conditions.

Here we report on the molecular identification, guard cell expression and functional characterization of AtGORK, an Arabidopsis thaliana guard cell outward rectifying K + channel. GORK represents a new member of the plant Shaker K + channel superfamily. When heterologously expressed in Xenopus oocytes the gene product of GORK mediated depolarization-activated K + currents. In agreement with the delayed outward rectifier in intact guard cells and protoplasts thereof, GORK is activated in a voltage-and potassium-dependent manner. Furthermore, the single channel conductance and regulation of GORK in response to pH changes resembles the biophysical properties of the guard cell delayed outward rectifier. Thus GORK very likely represents the molecular entity for depolarization-induced potassium release from guard cells. ß 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

It is generally accepted that K ؉ uptake into guard cells via inwardrectifying K ؉ channels is required for stomatal opening. To test whether the guard cell K ؉ channel KAT1 is essential for stomatal opening, a knockout mutant, KAT1::En-1, was isolated from an En-1 mutagenized Arabidopsis thaliana population. Stomatal action and K ؉ uptake, however, were not impaired in KAT1-deficient plants. Reverse transcription-PCR experiments with isolated guard cell protoplasts showed that in addition to KAT1, the K ؉ channels AKT1, AKT2͞3, AtKC1, and KAT2 were expressed in this cell type. In impalement measurements, intact guard cells exhibited inwardrectifying K ؉ currents across the plasma membrane of both wildtype and KAT1::En-1 plants. This study demonstrates that multiple K ؉ channel transcripts exist in guard cells and that KAT1 is not essential for stomatal action.

The potassium-channel gene, AKT3, has recently been isolated from an Arabidopsis thaliana cDNA library. By using the whole-mount and in situ hybridization techniques, we found AKT3 predominantly expressed in the phloem. To study the physiological role of this channel type, AKT3 was heterologously expressed in Xenopus oocytes, and the electrical properties were examined with voltage-clamp techniques. Unlike the plant inward-rectifying guard cell K ؉ channels KAT1 and KST1, the AKT3 channels were only weakly regulated by the membrane potential. Furthermore, AKT3 was blocked by physiological concentrations of external Ca 2؉ and showed an inverted pH regulation. Extracellular acidification decreased the macroscopic AKT3 currents by reducing the single-channel conductance. Because assimilate transport in the vascular tissue coincides with both H ؉ and K ؉ f luxes, AKT3 K ؉ channels may be involved in K ؉ transport accompanying phloem loading and unloading processes.The plant vascular system, which consists of xylem and phloem, is specialized for long-distance solute and water transport. In both tissues, potassium represents one of the major mineral nutrients and is likely to assist in osmotic homeostasis. After uptake from the soil, K ϩ ions circulate between roots and shoots through the xylem and phloem to adopt the specific demands for this cation in the various tissues (1, 2). Recently, in vivo and in vitro analysis demonstrated the presence of K ϩ uptake and release channels in xylem parenchyma cells (3-6). In comparison, information about K ϩ transport across the plasma membrane of phloem cells, the underlying mechanisms, and the physiological role in long-distance solute transport is still limited. Phloem loading with assimilates is accompanied by ionic movements (7). Protons are pumped into the apoplast by a H ϩ -ATPase, generating transmembrane gradients in electropotential and pH that in turn enable the uphill transport of assimilates into the phloem through assimilate/ H ϩ -cotransporters (8-10). The phloem loading coincides with an increase in the symplastic K ϩ concentration likely to maintain electrical neutrality that is required for creating the pH gradient (2, 7). In addition, the K ϩ concentration in the sieve tube may affect the volume flow rate in the phloem (11). Furthermore, the membrane potential that transiently changes during phloem-propagating action potentials is possibly reestablished on K ϩ release from the sieve tube (12). Evidence for the expression of K ϩ channels in the phloem was recently provided (P.A., K. Philippar, and R.H., unpublished data) to support the idea that K ϩ channels may also mediate the transmembrane K ϩ fluxes in the phloem.In the present paper, we localized the cloned Arabidopsis K ϩ channel AKT3 to the phloem and revealed its unique dependence on voltage, Ca 2ϩ , and pH properties, which are well suited for meeting its supposed role in processes associated with the phloem. MATERIALS AND METHODSRNA Extraction and Northern Blot Analysis. Total RNA was isolate...

Ion channels in roots allow the plant to gain access to nutrients. The composition of the individual ion channels and the functional contribution of different ␣-subunits is largely unknown. Focusing on K ؉ -selective ion channels, we have characterized AtKC1, a new ␣-subunit from the Arabidopsis shaker-like ion channel family. Promoter-␤-glucuronidase (GUS) studies identified AtKC1 expression predominantly in root hairs and root endodermis. Specific antibodies recognized AtKC1 at the plasma membrane. To analyze further the abundance and the functional contribution of the different K ؉ channels ␣-subunits in root cells, we performed real-time reverse transcription-PCR and patch-clamp experiments on isolated root hair protoplasts. Studying all shaker-like ion channel ␣-subunits, we only found the K ؉ inward rectifier AtKC1 and AKT1 and the K ؉ outward rectifier GORK to be expressed in this cell type. Akt1 knockout plants essentially lacked inward rectifying K ؉ currents. In contrast, inward rectifying K ؉ currents were present in AtKC1 knockout plants, but fundamentally altered with respect to gating and cation sensitivity. This indicates that the AtKC1 ␣-subunit represents an integral component of functional root hair K ؉ uptake channels.