The genomes of two nitrogen-fixing
Frankia
strains, Ag45/Mut15 and AgPM24, isolated from root nodules of
Alnus glutinosa
are described as representatives of a novel candidate species
.
Phylogenomic and ANI analyses confirmed that both strains are related to cluster 1 frankiae, and that both strains belong to a novel species. At 6.4 - 6.7 Mb, their genomes were smaller than those of other cultivated
Alnus
-infective cluster 1 strains but larger than that of the non-cultivated
Alnus
-infective cluster 1 Sp+ strain AgTrS that was their closest neighbor as assessed by ANI. Comparative genomic analyses identified genes essential for nitrogen-fixation, gene composition as regards COGs, secondary metabolites clusters and transcriptional regulators typical of those from
Alnus
-infective cluster 1 cultivated strains in both genomes. There were 459 genes present in other cultivated
Alnus
-infective strains lost in the two genomes, spread over the whole of the genome, which indicates genome erosion is taking place in these two strains.
The genomes of two nitrogen-fixing
Frankia
strains, AgB32 and AgKG'84/4, were isolated from spore-containing (spore+) and spore-free (spore-) root nodules of
Alnus glutinosa
, but they did not sporulate upon reinfection. The two strains are described as representatives of two novel candidate species
.
Phylogenomic and ANI analyses indicate that each strain represents a novel species within cluster 1, with genome sizes of 6.3 and 6.7 Mb smaller than or similar to those of other cultivated
Alnus
-infective cluster 1 strains. Genes essential for nitrogen-fixation, clusters of orthologous genes, secondary metabolite clusters and transcriptional regulators analyzed by comparative genomic analyses were typical of those from
Alnus
-infective cluster 1 cultivated strains in both genomes. Compared to other cultivated
Alnus
-infective strains with large genomes, those of AgB32 and AgKG'84/4 had lost 380 or 409 genes, among which one
hup
cluster, one
shc
gene and the
gvp
cluster, which indicates genome erosion is taking place in these two strains.
Here, we present the total genome sequence of Pantoea sp. strain paga, a plant-associated bacterium isolated from knots present on olive trees grown on the Adriatic Coast. The genome size of Pantoea sp. paga is 5.08 Mb, with a G+C content of 54%. The genome contains 4,776 predicted coding DNA sequences (CDSs), including 70 tRNA genes and 1 ribosomal operon. Obtained genome sequence data will provide insight on the physiology, ecology, and evolution of Pantoea spp.
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