Callisto®, containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. The objective of this work was to evaluate enzymatic and structural changes in Pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. Despite degradation of mesotrione, probably due a glutathione-S-transferase (GST) pathway in Pantoea ananatis, this herbicide induced oxidative stress by increasing hydrogen peroxide production. Thiol fragments, eventually produced after mesotrione degradation, could be involved in increased GST activity. Nevertheless, there was no peroxidation damage related to this production, as malondialdehyde (MDA) synthesis, which is due to lipid peroxidation, was highest in the controls, followed by the mesotrione- and Callisto®-treated cultures at log growth phase. Therefore, P. ananatis can tolerate and grow in the presence of the herbicide, probably due an efficient control of oxidative stress by a polymorphic catalase system. MDA rates depend on lipid saturation due to a pattern change to a higher level of saturation. These changes are likely related to the formation of GST-mesotrione conjugates and mesotrione degradation-specific metabolites and to the presence of cytotoxic adjuvants. These features may shift lipid membrane saturation, possibly providing a protective effect to bacteria through an increase in membrane impermeability. This response system in P. ananatis provides a novel model for bacterial herbicide tolerance and adaptation in the environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0240-x) contains supplementary material, which is available to authorized users.

The intense use of herbicides for weed control in agriculture causes selection pressure on soil microbiota and water ecosystems, possibly resulting in changes to microbial processes, such as biogeochemical cycles. These xenobiotics may increase the production of reactive oxygen species and consequently affect the survival of microorganisms, which need to develop strategies to adapt to these conditions and maintain their ecological functionality. This study analyzed the adaptive responses of bacterial isolates belonging to the same species, originating from two different environments (water and soil), and subjected to selection pressure by herbicides. The effects of herbicide Callisto and its active ingredient, mesotrione, induced different adaptation strategies on the cellular, enzymatic, and structural systems of two Bacillus megaterium isolates obtained from these environments. The lipid saturation patterns observed may have affected membrane permeability in response to this herbicide. Moreover, this may have led to different levels of responses involving superoxide dismutase and catalase activities, and enzyme polymorphisms. Due to these response systems, the strain isolated from water exhibited higher growth rates than did the soil strain, in evaluations made in oligotrophic culture media, which would be more like that found in semi-pristine aquatic environments. The influence of the intracellular oxidizing environments, which changed the mode of degradation of mesotrione in our experimental model and produced different metabolites, can also be observed in soil and water at sites related to agriculture. Since the different metabolites may present different levels of toxicity, we suggest that this fact should be considered in studies on the fate of agrochemicals in different environments.

Objective: to investigate the association between physical frailty and cognitive scores in older adults at an Open Universityof the Third Age in Southern Brazil. Methods: descriptive cross-sectional study with convenience sample comprising 100elderly, conducted from March to June 2013. For cognitive assessment, we applied the Mini Mental State Examination andthe Edmonton Frail Scale. Results: there was a predominance of females (93%), with a mean age of 65.6 years. 81% ofthe participants were classified as non-frail, 16% as apparently vulnerable to frailty, and 3% as mild frailty. There was asignificant association between cognitive performance and frailty (p<0.006). Conclusion: the research on the associationbetween physical frailty and cognitive scores in older people promotes the construction of gerontological care plans aimedat managing this syndrome.

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Factors associated with loss of handgrip strength in long-lived elderly RESUMEN Objetivo: Investigar la prevalencia de la reducción de la fuerza de agarre manual y los factores asociados en ancianos longevos, usuarios de la atención básica a la salud. Método: Estudio cuantitativo transversal, cuyos datos fueron recogidos en el período de enero a diciembre de 2013, por medio de pruebas y aplicación de cuestionarios. La muestra por conveniencia comprendió a 157 ancianos. Resultados: Señalan que la reducción de la fuerza de agarre manual tiene prevalencia moderada (25,5%), con predominio del sexo femenino (19,1%), del rango de edad de 80 a 89 años (18,5%) y baja escolaridad (15,9%). La asociación entre la disminución de la fuerza de agarre manual y las variables de edad e índice de masa corpórea mostró significación estadística. Conclusión: Las investigaciones acerca del componente de la fuerza de agarre manual son fundamentales para la identificación de las condiciones clínicas de los ancianos longevos brasileños y contribuyen para la construcción de planes dirigidos a la gestión de la fragilidad. DESCRIPTORES Anciano frágil Fuerza de la mano Enfermería geriátrica Salud del anciano RESUMOObjetivo: Investigar a prevalência da redução da força de preensão manual e fatores associados em idosos longevos, usuários da atenção básica de saúde. Método: Estudo quantitativo transversal, cujos dados foram coletados no período de janeiro a dezembro de 2013, por meio de testes e aplicação de questionários. A amostra por conveniência compreendeu 157 idosos. Resultados: Apontam que a redução da força de preensão manual possui prevalên-cia moderada (25,5%), com predomínio do sexo feminino (19,1%), da faixa etária de 80 a 89 anos (18,5%) e baixa escolaridade (15,9%). A associação entre diminuição da força de preensão manual e as variáveis idade e índice de massa corpórea mostrou significância estatística. Conclusão: As investigações sobre o componente da força de preensão manual são fundamentais para a identificação das condições clínicas dos idosos longevos brasileiros e contribuem para a construção de planos voltados para a gestão da fragilidade. DESCRITORES Idoso fragilizado Força da mão Enfermagem geriátrica Saúde do idoso ABSTRACTObjective: To investigate the prevalence of reduced grip strength and associated factors in long-lived elderly, who are users of primary health care. Method: Cross-sectional quantitative study, data were collected during the period of January to December of 2013, by applying tests and questionnaires. The convenience sampling was comprised of 157 seniors. Results: The findings indicate that the reduction in grip strength presents a moderate prevalence (25.5%), predominantly among females (19.1%), in the age group of 80-89 years (18.5%) and in those with lower educational levels (15.9%). The association between reduced grip strength and the variables of age and body mass index showed a statistical significance. Conclusion: Investigations about the handgrip strength are essential for identifying clinica...

RESUMO: Pesquisa transversal com o objetivo de investigar a associação entre fatores sociodemográficos e clínicos e os componentes força de preensão manual e velocidade da marcha em idosos longevos. A amostra contemplou 243 longevos, de três Unidades Básicas de Saúde de Curitiba, Paraná, entre janeiro de 2013 e setembro de 2015. Identificou-se associação significativa entre força de preensão manual e sexo (p=0,001), idade (p=0,001), estado civil (p=0,001), moradia (p=0,001), quedas no último ano (p=0,03), perda de urina (p=0,001), uso de bengala (p=0,001) e andador (p=0,001). A velocidade da marcha apresentou associação com sexo (p=0,001), idade (p=0,001), estado civil (p=0,001), moradia (p=0,001), quedas no último ano (p=0,03), perda de urina (p=0,001), uso de bengala (p=0,001) e andador (p=0,001). Destaca-se que a avaliação da força de preensão manual e velocidade da marcha auxiliam na identificação de alterações funcionais em longevos e constitui-se como parte fundamental na gestão da síndrome da fragilidade física. DESCRITORES: Idoso fragilizado; Força da mão; Marcha; Enfermagem geriátrica. FATORES SOCIODEMOGRÁFICOS E CLÍNICOS ASSOCIADOS SOCIODEMOGRAPHIC AND CLINICAL FACTORS ASSOCIATED WITH HAND GRIP STRENGTH AND GAIT SPEED IN LONG-LIVING ELDERLYABSTRACT: Cross-sectional research to investigate the association between sociodemographic and clinical factors and the components hand grip strength and gait speed in long-living elderly. The sample included 243 long-living elderly from three primary health care services in Curitiba, Paraná, between January 2013 and September 2015. A significant association was identified between hand grip strength and sex (p=0.001), age (p=0.001), marital status (p=0.001), housing (p=0.001), falls in previous year (p=0.03), urine loss (p=0.001), use of cane (p=0.001) and walker (p=0.001). The gait speed was associated with sex (p=0.001), age (p=0.001), marital status (p=0.001), housing (p=0.001), falls in previous year (p=0.03), urine loss (p=0.001), use of cane (p=0.001) and walker (p=0.001). It is highlighted that the assessment of hand grip strength and gait speed helps to identify functional changes in long-living elderly and plays a fundamental role in the management of the physical frailty syndrome.