Staphylococcus aureus is a common pathogen that causes various infections. Therefore, it is crucial to develop a fast and easy detection method for diagnosing and preventing S. aureus infections. In this study, MIRA assay was developed and validated (specificity; 100%) for the detection of S. aureus with nuc as the target gene. The reaction temperature and reaction time were then optimized, and the best reaction was at 40°C, 20 min. The assay could detect S. aureus in only 25 min. Additionally, the limit of detection of MIRA was 5 × 102 CFU/ml, 10-fold lower than that of the traditional PCR. Furthermore, this assay efficiently detected 219 S. aureus of 335 strains obtained from different bacterial samples (detection accuracy; 99.40%). In conclusion, this study provides a rapid and easy-to-operate method for the detection of S. aureus, and thus can be used for the timely diagnosis and prevention of S. aureus infection.
Common pathogenic
bacteria contaminate the environment through
various modes of transmission. It is thus crucial to develop simple
preparation methods of residue-free environmental disinfectants. β-Lactam
antibiotics are frequently prescribed in clinical practice to treat
bacterial infections. In this study, we used electrochemical exfoliation
to synthesize graphene oxide (GO) with abundant ketene functional
groups. A residue-free GO−β-lactam (GOβL) was subsequently
obtained by mixing ketene and azomethine-H via a [2 + 2] cycloaddition
reaction in the aqueous phase. GOβL has shown broad-spectrum
bacterial inhibition against four bacteria (
Staphylococcus
aureus
,
Escherichia coli
,
Salmonella enterica
, and
Shigella dysenteriae
), and it degrades rapidly within
24 h. This study provides a fast and easy method for the synthesis
of GOβL, which can be employed as a promising environmental
bacteriostatic disinfectant in real-life applications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.