Penfluridol, a commonly used antipsychotic agent in a clinical setting, exhibits potential anticancer properties against various human malignancies. Here, we investigated the effect of penfluridol on the biological behavior of colorectal cancer (CRC) cells. Cell viability and clonogenic potential were detected by the cell counting kit‐8 and colony formation assay. The cell apoptosis and cell cycle distribution were quantified through flow cytometry. Caspase‐3 activity, glucose consumption, lactate production, and intracellular ATP levels were evaluated using the corresponding commercial detection kits. The protein levels of related genes were detected through western blotting. Mitochondrial membrane potential was detected using JC‐1 staining. A CRC xenograft tumor model was used to validate the antitumor activity of penfluridol in vivo. Penfluridol reduced cell survival and promoted apoptotic cell death effectively through the mitochondria‐mediated intrinsic pathway in a dose‐dependent manner. Furthermore, the process of glycolysis in HCT‐116 and HT‐29 cells was inhibited upon penfluridol treatment, as evidenced by the decrease in glucose consumption, lactate production, and intracellular ATP levels. Further mechanistic studies revealed that penfluridol influenced cell apoptosis and glycolysis in CRC cells by downregulating hexokinase‐2 (HK‐2). The proapoptotic effect and glycolytic inhibition‐induced by penfluridol were effectively reversed by HK‐2 overexpression. Consistent with in vitro results, penfluridol could also suppress tumor growth and trigger apoptosis in vivo. Penfluridol triggers mitochondrial‐mediated apoptosis and induces glycolysis inhibition via modulating HK‐2 in CRC and provides a theoretical basis to support penfluridol as a repurposed drug for CRC patients.
Aims To examine the perceived professional benefits (PPB) and associated factors among nurses during the coronavirus disease 2019 (COVID‐19) pandemic in China. Design Cross‐sectional study. Methods Using the snowball sampling method, 492 nurses (478 females, 14 males) were recruited. Data were collected using an online survey, including participants' socio‐demographic and working characteristics, psychological distress related to the COVID‐19 pandemic, dealing with professional frustration, professional self‐reflection and PPB from 1–30 April 2020. Results Nurses experienced high levels of PPB. In linear regression analysis, self‐perceived concerns about COVID‐19, emotional shock caused by it, risk perception towards their occupations, dealing with professional frustration and professional self‐reflection were positively associated with PPB among nurses. These factors explained 84% variance in PPB. Conclusions This study highlighted that although the nurses experienced psychological distress, they gained high PPB during the COVID‐19 pandemic. Additionally, to facilitate nurses' efforts to achieve professional growth, more educational resources and opportunities for engaging in reflective practices could be provided.
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