Background and Purpose: Cytomegalovirus causes asymptomatic disease in individuals with normal immune system; and leads to serious complications in immunocompromised individuals and fetus. In CMV, gB is the most studied glycoprotein in terms of genotyping. Up to now, four different gB genotypes (gB 1-4) of CMV have been identified.
In this study, it was aimed to determine the genotypes of CMV strains obtained from patients with immune deficiency.
Methods: Twenty children and 29 adults, 49 patients who were followed in the Department of Adult Hematology and Pediatric Hematology were included in the study. DNA isolation was performed from samples with CMV DNA level of 1000 IU / ml and above, and 474 bp region from the gB region of the virus was amplified by nested PCR. This region was sequenced by the Sanger (ABI 3500 Prism) sequencing. Next generation sequencing (NGS) method was applied to the samples that CMV genotype could not be determined by Sanger sequencing.
Results: Distribution of CMV genotypes of patients determined by Sanger sequencing; while it was determined as 18/49 (36.7%) type 1, 5/49 (10.2%) type 2, 5/49 (10.2%) type 3 and 1/49 (2%) type 4; 14/49 (28.5%) of them were found as mixed genotypes. CMV genotype could not be determined in 6 patients by Sanger sequencing and CMV genotype of these 6 patients were found as mixed genoype by NGS. Mixed genotype was detected in 20 (40.9%) of 49 patients, in total by Sanger sequencing and NGS.
Conclusion: It is remarkable that the most common genotype is mixed genotype. It was concluded that it is appropriate to study the next generation sequencing method in patients whose CMV genotype cannot be determined by Sanger sequencing.
Çalışmada lisans sağlık programlarında okuyan öğrencilerin HPV enfeksiyonu ve HPV aşıları hakkındaki temel bilgi ve tutumlarını değerlendirmek amaçlanmıştır. Çalışma 25.04.2022-14.06.2022 tarihleri arasında Nevşehir’de öğrenim gören 238 Diş Hekimliği Fakültesi ve Sağlık Bilimleri Yüksekokulu öğrencileri ile kesitsel olarak yapılmıştır. Anket dijital ortamda cevaplanmış olup sosyodemografik veriler, HPV enfeksiyon bilgisi, HPV aşı bilgisi, HPV aşılarına karşı tutum bölümlerinden oluşmaktadır. Katılımcıların 186’sı kadın (%78.2), ortalama yaş 20.9 (min:18; max:33, ss:1.65)’dur. Rahim ağzı kanseri ile HPV enfeksiyonu ilişkisini bilenler %84 olup kadınların %86’sının, erkeklerin %82.6’sının HPV aşısının rahim ağzı kanserine karşı koruyuculuğuna inandığı görülmüştür. Aşının güvenirliğini kabul eden kadınların oranı %47.8 (89/186), erkeklerin oranı %34.6 (18/52)’dır.
Çalışmada ortalama puan 16.82 olup katılımcıların bilgi düzeyi ‘yeterli’ olarak bulunmuştur. Araştırmada HPV bilgi düzeyi yeterli olan kadınların oranı %51.6, erkeklerin oranı %12.8 şeklindedir. Katılımcıların bilgi düzeyleri ile cinsi¬yetleri arasında anlamlı bir ilişki bulunmazken (p=0.925), okudukları programlar ile bilgi düzeyleri arasında istatistik¬sel olarak anlamlı bir ilişki bulunmuştur (p
Investigation of Nasal Methicillin Resistant Staphylococcus aureus and Coagulase Negative Staphylococcus Carriage by Various Methods in Hemodialysis Patients in Nevşehir ProvinceInfection is one of the important factors affecting morbidity and mortality in hemodialysis patients. Access to dialysis, frequent hospitalizations, and contact with healthcare personnel increase the risk of infection. Nasal Staphylococcus aureus and other staphylococci may cause vascular access infections through carrier patients and staff. In this study, nasal S. aureus and coagulasenegative Staphylococcus (CNS) carriers were detected in hemodialysis patients and staff, and methicillin resistance in isolates was investigated by various methods. In this way, it was aimed to contribute to the assessment of nosocomial infection risk. Between November 2019 and March 2020, 93 hemodialysis patients, 41 female, 52 male, and 15 dialysis personnel treated at Nevşehir State Hospital, H. Mustafa and Türkan Öbekli Dialysis Center were included in the study. Nasal swab samples were obtained after the informed consent form was signed. Methicillin resistance in staphylococci isolated from the samples was determined by the Kirby-Bauer disk diffusion method, PBP-2a latex agglutination (LA) test, and real-time polymerase chain reaction (RT-PCR). Of the patient samples (n=93), 18 (19.3%) had S. aureus, 11 (11.8%) had CNS; of the personnel samples (n=15) CNS was isolated from three (20%) and S. aureus from one (6.6%). It was determined that one of the S. aureus isolated from the patients was methicillin-resistant (1%); the rest (18.2%) and all CNS (11.8%) were methicillin-susceptible by disk diffusion method. While all of the CNS (20%) in the personnel samples were susceptible to methicillin, only S. aureus was resistant (6.6%). The PBP-2a LA test and RT-PCR were performed on all S. aureus isolates (n=19); the PBP-2a
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