SummaryB. subtilis a-amylase was immobilized on cyanogen bromide activated carboxymethyl cellulose. The conversion of wheat starch was carried out at 72°C in a stirred tank by soluble and immobilized a-amylase. The initial reaction rate with immobilized a-amylase was lower than with the soluble enzyme, but after 1 hr immobilized a-amylase produced a higher quantity of reducing sugars than the soluble enzyme. The action pattern of immobilized a-amylase was different from that of the soluble enzyme: immobilized a-amylase produced relatively more glucose and maltose, except a t the beginning of conversion. Immobilized a-amylase readily hydrolyzed G6. The starch conversion by immobilized a-amylase was not diffusion controlled a t a stirring rate of 100-300 rpm.
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