Introduction: In senile osteoporosis countering the age-mediated bone loss, promotion of osteoblastogenesis and identification of responsible micro-RNA (miR) would be a successful strategy. Material and methods: miR microarray screening was carried out to identify the suppressed miRs after real time polymerase chain reaction (RT-PCR) analysis in mesenchymal stem cells (MSCs) derived from adult bone marrow during the proliferation to the mineralization stage. The primary calvarial pre-osteoblasts (human) were harvested and received transfection of miR-22's antagomir or agomir in vitro. Bioinformatics study suggested YWHAZ as the favorable target gene. Next, YWHAZ knockdown was studied for its effect on differentiation of osteoblasts. For in vivo studies, ovariectomized or sham mice were injected with miR-22's antagomir for a period of 6 weeks. The stromal cells were isolated in the 6 th week for ex vivo experiments. Results: miR-22 was found to be down-regulated in bone marrow derived mesenchymal stem cells. miR-22's antagomir converted the pre-osteoblasts to a more differentiated and mineralized phenotype showing upregulated protein expression of COL1A1, ALP and CBFA1. The miR-22's antagomir suppressed YWHAZ, enhanced stability of CBFA1 and promoted the differentiation of osteoblasts. In vivo, miR-22's antagomir promoted mineralization and osteoblastogenesis, elevated bone strength and reversed the ovariectomy mediated bone loss in sham mice. Conclusions: Inhibition of miR-22 may be a potential target for treating osteoporosis clinically. The findings hence suggest that inhibition of miR-22 may be an effective anabolic therapeutic approach in treating osteoporosis clinically.
In this work, a branched peptide amphiphile (B-PA) presenting RGD and IKVAV motifs was fabricated by solid-phase peptide synthesis and self-assembled into a nanofiber hydrogel in which rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded and cultured for seven days. Specifically, 1 wt% B-PA was self-assembled into a nanofiber hydrogel with the addition of culture medium and observed using transmission electron microscopy. The B-PA with a molecular weight of 2191.72 and a purity >95% self-assembled into nanofibers with diameters from 6 to 8 nm and lengths ranging from hundreds of nanometers to several micrometers. BMSCs were acquired from rabbits using differential adherence methods and identified by flow cytometry for cell phenotype. The cells were stained with calcein acetoxymethyl ester/propidium iodide to assess cell viability, CCK-8 to assess cell cytotoxicity and proliferation, and Hochest 33342 to assess cell adhesion. They were also immunofluorescently labeled with microtubule-associated protein-2 (MAP-2), neurofilament protein (NF), and glial fibrillary acidic protein (GFAP) to assess cell transdifferentiation. The B-PA hydrogel provided platform upon which the CD29+/44+ cells adhered and proliferated, and it induced the transdifferentiation of cells into neural cells expressing the markers MAP-2, NF, and GFAP. The hydrogel exhibited good cytocompatibility and multiple functions, and may therefore serve as a scaffold for neural tissue engineering.
Background: Spinal cord injury (SCI) remain one of the great clinical challenges because of their considerable long-term disability potential. In this study, A branched amphiphilic peptide (B-PA) segments were constructed and self-assembled into hydrogels to support bone marrow derived mesenchymal stromal cells (BMSCs) encoding Recombinant Rat VEGF165(VEGF165), neurotrophins-3(NT-3)and angiopoietin-1(ANG-1) genes. In addition, B-PAS promoted the transdifferentiation of BMSCs into nerve and vascular endothelial cells. Methods:Self-assembly of b-PA into a nanofiber hydrogel was triggered by the culture medium and analysed by transmission electron microscopy (TEM). Rat BMSCs were cultivated using the differential adhesion method and assessed by flow cytometry. Cells were inoculated into the hydrogel and investigated using calcein-AM/PI, CCK-8, and cell adhesion assays. Cells transfected with adenoviral vectors of biocarrier (Adv-Bic) and adenoviral vectors of NT-3 gene (AdvNT-3) were cultured for 7 days and evaluated by immunofluorescence and real time quantitative reverse transcription-PCR. Results:The molecular weight and purity of b-PA were 2191.72 and >95%, respectively. Self-assembled nanofibers had a diameter of 5-8 nm and a length ranging from hundreds of nanometres to micrometres. The resulting CD29+/44+ cells could differentiate into osteoblasts and fat cells, and the hydrogel supported cell growth, proliferation and adhesion. Cells in the hydrogel overexpressed VEGF165, NT-3 and Ang-1, and NSE and Nestin markers of CD31+/34+ cells were also abundant. Conclusions: The hydrogel has potential as a tissue engineering scaffold with good cytocompatibility, and can induce genetically modified BMSCs to transdifferentiate into neural and endothelial cell (EC)-like cells.
Purpose. In this study, a systematic review and meta-analysis were used to examine the effectiveness of nursing care in the treatment of bladder cancer patients. The platforms of PubMed, Embase, Cochrane Library, and Web of Science were used to conduct a thorough literature search. Methods. The searching approach was used to find the fundamental characteristics of 5 studies. Sample size ranged from 52 to 131,852, and total sample size was 151,166. The study was looked up in PubMed, Embase, and Web of Science, with the most recent search being done in July 2022. Utilizing a standardized form, two independent reviewers gathered pertinent information from research that qualified as literature (17). Review Manager 5.3 used the data to examine the literature. Statistics were deemed significant at p < 0.05 . Results. We discovered that more bladder cancer patients with T1+T2 tumor stages were receiving nursing care than those with T1+T2 tumor stages were receiving control care (mean difference =1.27, 95% CI: 1.20-1.35, p < 0.00001 ). The proportion of bladder cancer patients with T3+T4 tumor stage in the nursing care group was lower than the proportion of patients with T3+T4 tumor stage in the control group (mean difference = 1.07; 95% CI: 1.01-1.14; p < 0.00001 ). The difference between the number of bladder cancer patients receiving radiotherapy in the nursing care group and the control group was not statistically significant (mean difference = 1.07, 95% confidence interval [CI]: 0.99-1.16, p = 0.11 ). There were fewer patients with bladder cancer receiving chemotherapy in the nursing care group than that in the control group (mean difference = -0.02, 95% CI: -0.0-0.02, p < 0.00001 ). The incidence rate of patients with bladder cancer with major complications in nursing care group was lower than that of patients with bladder cancer with major complications in control group (mean difference = 0.41 95% CI: 0.18-0.93, p = 0.03 ). When compared to patients with bladder cancer who had serious complications in the control group, the hospital death rate for nursing care patients had a greater incidence of bladder cancer patients (mean difference = 4.64 95% CI: 4.46-4.82, p < 0.00001 ). Conclusion. This study demonstrated that the effects of nursing care reduced the incidence rate of chemotherapy and the frequency of severe problems in bladder cancer patients.
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