Circular RNAs (circRNAs) have confirmed to participate in diverse biological functions in cancer. However, the expression patterns of circRNAs on hepatocellular carcinoma (HCC) remains unclear. In the present study, we clarified that hsa_circRNA_104348 was dramatically upregulated in HCC tissues and cells. Patients with HCC displaying high hsa_circRNA_104348 level possessed poor prognosis. Has_circ_104348 facilitated proliferation, migration, and invasion, meanwhile suppressed apoptosis of HCC cell. Furthermore, hsa_circRNA_104348 directly targeted miR-187–3p, could regulate miR-187-3p to affect proliferation, migration, invasion, and apoptosis of HCC cells, and may have effect on Wnt/β-catenin signaling pathway. Moreover, RTKN2 could be a direct target of miR-187-3p. In addition, knockdown of hsa_circRNA_104348 attenuated HCC tumorigenesis and lung metastasis in vivo. Taken together, these findings indicated that circular RNA hsa_circRNA_104348 might function as a competing endogenous RNA (ceRNA) to promotes HCC progression by targeting miR-187–3p/RTKN2 axis and activating Wnt/β-catenin pathway.

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Severe hand-foot-and-mouth disease (HFMD) caused by Enterovirus 71 (EV71) always accompanies with inflammation and neuronal damage in the central nervous system (CNS). During neuronal injuries, cell surface-exposed calreticulin (Ecto-CRT) is an important mediator for primary phagocytosis of viable neurons by microglia. Our data confirmed that brainstem neurons underwent neuronophagia by glia in EV71-induced death cases of HFMD. EV71 capsid proteins VP1, VP2, VP3, or VP4 did not induce apoptosis of brainstem neurons. Interestingly, we found VP1-activated endoplasmic reticulum (ER) stress and autophagy could promote Ecto-CRT upregulation, but ER stress or autophagy alone was not sufficient to induce CRT exposure. Furthermore, we demonstrated that VP1-induced autophagy activation was mediated by ER stress. Meaningfully, we found dexamethasone treatment could attenuate Ecto-CRT upregulation by alleviating VP1-induced ER stress. Altogether, these findings identify VP1-promoted Ecto-CRT upregulation as a novel mechanism of EV71-induced neuronal cell damage and highlight the potential of the use of glucocorticoids to treat severe HFMD patients with CNS complications.

Taking a hybrid energy storage system (HESS) composed of a battery and an ultracapacitor as the study object, this paper studies the energy management strategy (EMS) and optimization method of the hybrid energy storage system in the energy management and control strategy of a pure electric vehicle (EV) for typical driving cycles. The structure and component model of the HESS are constructed. According to the fuzzy control strategy, aimed at the roughness of the membership function in EMS, optimization strategies based on a genetic algorithm (GA) and particle swarm optimization (PSO) are proposed; these use energy consumption as their optimal objective function. Based on the improved EV model, the fuzzy control strategy is studied in MATLAB/Advisor, and two control strategies are obtained. Compared with the simulation results based on three driving cycles, urban dynamometer driving schedule (UDDS), new European driving cycle (NEDC), and ChinaCity, the optimum control strategy were obtained. The theoretical minimum energy consumption of HESS was reached by dynamic programming (DP) algorithm in the same simulation environment. The research shows that, compared with the PSO, the output current peak and current fluctuation of the battery optimized by the GA are lower and more stable, and the total energy consumption is reduced by 3–9% in various simulation case studies. Compared with the theoretical minimum value, the deviation of energy consumption simulated by GA-Fuzzy Control is 0.6%.