Polymer-based bioresorbable scaffolds (BRS) seek to eliminate long-term complications of metal stents. However, current BRS designs bear substantially higher incidence of clinical failures, especially thrombosis, compared with metal stents. Research strategies inherited from metal stents fail to consider polymer microstructures and dynamics--issues critical to BRS. Using Raman spectroscopy, we demonstrate microstructural heterogeneities within polymeric scaffolds arising from integrated strain during fabrication and implantation. Stress generated from crimping and inflation causes loss of structural integrity even before chemical degradation, and the induced differences in crystallinity and polymer alignment across scaffolds lead to faster degradation in scaffold cores than on the surface, which further enlarge localized deformation. We postulate that these structural irregularities and asymmetric material degradation present a response to strain and thereby clinical performance different from metal stents. Unlike metal stents which stay patent and intact until catastrophic fracture, BRS exhibit loss of structural integrity almost immediately upon crimping and expansion. Irregularities in microstructure amplify these effects and can have profound clinical implications. Therefore, polymer microstructure should be considered in earliest design stages of resorbable devices, and fabrication processes must be well-designed with microscopic perspective.

No abstract

Drug eluting stents are associated with late stent thrombosis (LST), delayed healing and prolonged exposure of stent struts to blood flow. Using macroscale disturbed and undisturbed fluid flow waveforms, we numerically and experimentally determined the effects of microscale model strut geometries upon the generation of prothrombotic conditions that are mediated by flow perturbations. Rectangular cross-sectional stent strut geometries of varying heights and corresponding streamlined versions were studied in the presence of disturbed and undisturbed bulk fluid flow. Numerical simulations and particle flow visualization experiments demonstrated that the interaction of bulk fluid flow and stent struts regulated the generation, size and dynamics of the peristrut flow recirculation zones. In the absence of endothelial cells, deposition of thrombin-generated fibrin occurred primarily in the recirculation zones. When endothelium was present, peristrut expression of anticoagulant thrombomodulin (TM) was dependent on strut height and geometry. Thinner and streamlined strut geometries reduced peristrut flow recirculation zones decreasing prothrombotic fibrin deposition and increasing endothelial anticoagulant TM expression. The studies define physical and functional consequences of macro-and microscale variables that relate to thrombogenicity associated with the most current stent designs, and particularly to LST.

Surface erosion and underground leakage loss simultaneously exist on slopes with a double‐layered structure (surface and underground) and have caused rocky desertification in karst regions. Because of the great difficulty of directly determining the underground leakage loss of soil and water, soil erosion processes in this region remain unclear, especially underground leakage loss. The aim of this study was to reveal the plot‐scale characteristics of surface soil erosion and underground leakage loss of yellow soils on karst slopes. Simulated rainfall tests and field runoff plot monitoring were conducted to achieve this aim. We found that surface erosion formed on steep slopes (30°) or at greater rainfall intensities (over 50 mm hr−1) and that surface runoff and surface soil loss dominated the total runoff and soil loss on karst slopes at greater rainfall intensities. However, underground leakage loss always occurred at different slopes and rainfall intensities. For small rainfall intensity cases (lower than 80 mm hr−1), underground runoff and underground soil leakage loss dominated the total runoff and soil loss and showed an underground runoff ratio of over 90% and an underground soil leakage ratio of over 44%. Rainfall intensity had significant effects on surface runoff depth and surface soil loss rates but an insignificant influence on underground leakage loss. Slope gradient and slope length influenced the runoff yield, and slope degree had a greater effect on the surface soil loss than had slope length. This study improves our understanding of surface erosion and underground leakage loss in karst rocky desertification regions.

Polymeric bioresorbable scaffolds (BRS), at their early stages of invention, were considered as a promising revolution in interventional cardiology. However, they failed dramatically compared to metal stents showing substantially higher incidence of device failure and clinical events, especially thrombosis. One problem is that use of paradigms inherited from metal stents ignores dependency of polymer material properties on working environment and manufacturing/deployment steps. Unlike metals, polymeric material characterization experiments cannot be considered identical under dry and submerged conditions at varying rates of operation. We demonstrated different material behaviors associated with variable testing environment and parameters. We, then, have employed extracted material models, which are verified by computational methods, to assess the performance of a full-scale BRS in different working condition and under varying procedural strategies. Our results confirm the accepted notion that slower rate of crimping and inflation can potentially reduce stress concentrations and thus reduce localized damages. However, we reveal that using a universal set of material properties derived from a benchtop experiment conducted regardless of working environment and procedural variability may lead to a significant error in estimation of stress-induced damages and overestimation of benefits procedural updates might offer. We conclude that, for polymeric devices, microstructural damages and localized loss of structural integrity should complement former macroscopic performance-assessment measures (fracture and recoil). Though, to precisely capture localized stress concentration and microstructural damages, context-related testing environment and clinically-relevant procedural scenarios should be devised in preliminary experiments of polymeric resorbable devices to enhance their efficacy and avoid unpredicted clinical events.

Rapid urbanization has led to dramatic changes in urban forest structures and functions, and consequently affects carbon (C) storage in cities. In this study, field surveys were combined with high resolution images to investigate the variability of C storage of urban forests in Changchun, Northeast China. The main objectives of this study were to quantify the C storage of urban forests in Changchun City, Northeast China and understand the effects of forest type and urbanization on C storage of urban forests. The results showed that the mean C density and the total C storage of urban forests in Changchun were 4.41 kg/m 2 and 4.74 × 10 8 kg, respectively. There were significant differences in C density among urban forest types. Landscape and relaxation forest (LF) had the highest C density with 5.41 kg/m 2 , while production and management forest (PF) had the lowest C density with 1.46 kg/m 2. These differences demonstrate that urban forest type is an important factor needed to be considered when the C storage is accurately estimated. Further findings revealed significant differences in different gradients of urbanization, and the mean C density decreased from the first ring (6.99 kg/m 2) to the fourth ring (2.87 kg/m 2). The total C storage increased from the first ring to the third ring. These results indicate that C storage by urban forests will be significantly changed during the process of urbanization. The results can provide insights for decision-makers and urban planners to better understand the effects of forest type and urbanization on C storage of urban forests in Changchun, and make better management plans for urban forests.

Urban forest soil infiltration, affected by various factors, is closely related with surface runoff. This paper studied the effect of urban forest types, vegetation configuration and soil properties on soil infiltration. In our study, 191 typical plots were sampled in Changchun City, China to investigate the soil infiltration characteristics of urban forest and its influencing factors. Our results showed that the steady infiltration rates of urban forest soil were highly variable. High variations in the final infiltration rates were observed for different vegetation patterns and compaction degrees. Trees with shrubs and grasses had the highest infiltration rate and trees with bare land had the lowest infiltration rate. In addition, our results showed that the soil infiltration rate decreased with an increase in the bulk density and with a reduction in the soil organic matter content and non-capillary porosity. The soil infiltration rate also had significantly positive relationships with the total porosity and saturated soil water content. Urban soil compaction contributed to low soil infiltration rates. To increase the infiltration rate and water storage volume of urban forest soil, proper techniques to minimize and mitigate soil compaction should be used. These findings can provide useful information for urban planners about how to maximize the water volume of urban forest soil and decrease urban instantaneous flooding.

No abstract