Background/Aims: IgA nephropathy (IgAN) with isolated microscopic hematuria (IMH) is prevalent in Asian countries including China. However, the natural history of IgAN with IMH has not yet been clarified. The aim of this study was to review the natural course and prognostic factors of IgAN with IMH in Chinese patients. Methods: We retrospectively studied 135 patients (43 males and 92 females) followed up for a mean period of 92 ± 28 months. In order to identify factors associated with renal progression, clinical and pathological data at onset were reviewed. Results: During the follow-up period, hematuria of 16 patients (12%) disappeared while persistent microscopic hematuria was seen in 119 patients (88%), and proteinuria was present in 39 patients (29%). The prevalence of hypertension was 32% (43 patients), and 20% (27 patients) developed renal insufficiency. The prevalence of proteinuria and hypertension in the microalbuminuria group was significantly higher than those in the normoalbuminuria group. Poor renal outcome is usually associated with hematuria, microalbuminuria, and tubulointerstitial lesions. Conclusion: IgAN with IMH may not imply favorable outcome, so early diagnosis and careful follow-up are clinically significant. Hematuria, microalbuminuria, and tubulointerstitial lesions are useful markers to identify those patients at high risk for renal progression.
Context
Diabetic kidney disease (DKD) is a devastating complication of diabetes. Renal functional deterioration caused by tubular injury is the primary change associated with this disease. Calycosin shows protective roles in various diseases.
Objectives
This study explored the function and underlying mechanism of calycosin in DKD.
Materials and methods
HK-2 cells were treated with 25 mM high glucose (HG) to establish a renal tubule injury cell model. Then, the viability of cells treated with 0, 5, 10, 20, 40 and 80 μM of calycosin was measured using Cell Counting Kit-8. For the
in vivo
model, db/db mice were treated with 10 and 20 mg/kg/day of calycosin; db/m mice served as controls. The histomorphology was analyzed via haematoxylin and eosin staining.
Results
HG-induced decreased expression of glutathione (491.57 ± 33.56 to 122.6 ± 9.78 μmol/mL) and glutathione peroxidase 4 (inhibition rate 92.3%) and increased expression of lactate dehydrogenase (3.85 ± 0.89 to 16.84 ± 2.18 U/mL), malondialdehyde (3.72 ± 0.66 to 18.2 ± 1.58 nmol/mL), lipid ROS (4.31-fold increase) and NCOA4 (7.69-fold increase). The effects induced by HG could be blocked by calycosin. Moreover, calycosin alleviated the HG-induced decrease of cell viability and the increase of lipid ROS, but erastin could block the effects caused by calycosin. The
in vivo
model showed that calycosin alleviated the renal injury caused by diabetes.
Discussion and conclusion
Calycosin has a protective effect on diabetic kidney disease; ferroptosis may be involved in this process.
The results indicate that KQL is able to protect renal function via ameliorating experimental rat renal failure as found in these renal functional parameters.
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