The ability to detect moving objects is an ethologically salient function. Direction-selective neurons have been identified in the retina, thalamus, and cortex of many species, but their homology has remained unclear. For instance, it is unknown whether direction-selective retinal ganglion cells (DSGCs) exist in primates and, if so, whether they are the equivalent to mouse and rabbit DSGCs. Here, we used a molecular/circuit approach in both sexes to address these issues. In mice, we identify the transcription factor Satb2 (special AT-rich sequence-binding protein 2) as a selective marker for three RGC types: On-Off DSGCs encoding motion in either the anterior or posterior direction, a newly identified type of Off-DSGC, and an Off-sustained RGC type. In rabbits, we find that expression of Satb2 is conserved in On-Off DSGCs; however, it has evolved to include On-Off DSGCs encoding upward and downward motion in addition to anterior and posterior motion. Next, we show that macaque RGCs express Satb2 most likely in a single type. We used rabies virus-based circuitmapping tools to reveal the identity of macaque Satb2-RGCs and discovered that their dendritic arbors are relatively large and monostratified. Together, these data indicate Satb2-expressing On-Off DSGCs are likely not present in the primate retina. Moreover, if DSGCs are present in the primate retina, it is unlikely that they express Satb2.
The
adsorption properties of methane (CH4) have a great
influence on shale gas exploration and development. The surface chemistry
characteristics of nanopores are key factors in adsorption phenomena.
The clay pores in shale formations exhibit basal surface and edge
surfaces (mainly as A and C chain and B chain surfaces in illite).
Little research regarding CH4 adsorption on clay edge surfaces
has been carried out despite their distinct surface chemistries. In
this work, the adsorption of CH4 confined in nanoscale
illite slit pores with basal and edge surfaces was investigated by
grand canonical Monte Carlo and molecular dynamics simulations. The
adsorbed phase density, adsorption capacity, adsorption energy, isosteric
heat of adsorption, and adsorption sites were calculated and analyzed.
The simulated adsorption capacity compares favorably with the available
experimental data. The results show that the edge surfaces have van
der Waals interactions that are weaker than those of the basal surfaces.
The adsorption capacity follows the order basal surface > B chain
surface > A and C chain surface. However, the differences of adsorption
capacity between these surfaces are small; thus, edge surfaces cannot
be ignored in shale formation. Additionally, we confirmed that the
adsorbed phase has a thickness of approximately 0.9 nm. The pore size
determines the interaction overlap strength on the gas molecules,
and the threshold value of the pore size is about 2 nm. The preferential
adsorption sites locate differently on edge and basal surfaces. These
findings could provide deep insights into CH4 adsorption
behavior in natural illite-bearing shales.
Purpose Management of vestibular schwannoma (VS) is based on tumour size as observed on T1 MRI scans with contrast agent injection. The current clinical practice is to measure the diameter of the tumour in its largest dimension. It has been shown that volumetric measurement is more accurate and more reliable as a measure of VS size. The reference approach to achieve such volumetry is to manually segment the tumour, which is a time intensive task. We suggest that semi-automated segmentation may be a clinically applicable solution to this problem and that it could replace linear measurements as the clinical standard. Methods Using high-quality software available for academic purposes, we ran a comparative study of manual versus semiautomated segmentation of VS on MRI with 5 clinicians and scientists. We gathered both quantitative and qualitative data to compare the two approaches; including segmentation time, segmentation effort and segmentation accuracy. Results We found that the selected semi-automated segmentation approach is significantly faster (167 s vs 479 s, p < 0.001), less temporally and physically demanding and has approximately equal performance when compared with manual segmentation, with some improvements in accuracy. There were some limitations, including algorithmic unpredictability and error, which produced more frustration and increased mental effort in comparison with manual segmentation. Conclusion We suggest that semi-automated segmentation could be applied clinically for volumetric measurement of VS on MRI. In future, the generic software could be refined for use specifically for VS segmentation, thereby improving accuracy.
Water always occurs in gas shales, especially during the treatment of shale gas hydraulic fracturing. In sharp contrast to the prevailing view that water film is ubiquitous in shale formations, we observed an unusual phenomenon that water bridge instead of water film dominates in some illite and kaolinite slit pores when we are investigating the coexisting pattern of water and methane inside shale nanopores using molecular dynamics simulations. The network orientation structure and hydrogen bond of water molecules are analyzed, and the results indicate that appearance of water bridge is attributed to the strong internal, self-generated electric field induced by surface charge contrast between different pore surfaces. Four factors can significantly influence this self-generated electric field strength: pore surface chemistry, mineral type, pore shape, and pore size. When the pore size is within several nanometers, a small charge difference could induce strong electric field and change the structural properties of water clusters. The water film or water bridge inside shale nanopores alters the hydraulic diameter of the pore and the fluid flow pattern. These findings may provide a better and microscopic insight of the water−gas flow behavior and the electric field inside clay nanopores.
Binocular disparity information is an important source of 3D perception. Neurons sensitive to binocular disparity are found in almost all major visual areas in nonhuman primates. In area V4, disparity processes are suggested for the purposes of 3D-shape representation and fine disparity perception. However, whether neurons in V4 are sensitive to disparity-defined edges used in shape representation is not clear. Additionally, a functional organization for disparity edges has not been demonstrated so far. With intrinsic signal optical imaging, we studied functional organization for disparity edges in the monkey visual areas V1, V2, and V4. We found that there is an orientation map in V4 activated by edges purely defined by binocular disparity. This map is consistent with the orientation map obtained with regular luminance-defined edges, indicating a cue-invariant edge representation in this area. In contrast, such a map is much weaker in V2 and totally absent in V1. These findings reveal a hierarchical processing of 3D shape along the ventral pathway and the important role that V4 plays in shape-from-disparity detection.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.