In this article, a stock-forecasting model is developed to analyze a company's stock price variation related to the Taiwanese company HTC. The main difference to previous articles is that this study uses the data of the HTC in recent ten years to build a Markov transition matrix.Instead of trying to predict the stock price variation through the traditional approach to the HTC stock problem, we integrate two types of Markov chain that are used in different ways. One is a regular Markov chain, and the other is an absorbing Markov chain. Through a regular Markov chain, we can obtain important information such as what happens in the long run or if the distribution of the states tends to stabilize over time in an efficient way. Next, we used an artificial variable technique to create an absorbing Markov chain. Thus we used an absorbing Markov chain to provide information about the period between the increases before arriving at the decreasing state of the HTC stock. We provide investors with information on how long the HTC stock will keep increasing before its price begins to fall, which is extremely important information to them.
Bacillus cereus is a major food-born pathogen in Taiwan and its major syndromes include vomiting, fever and diarrhea. To minimize the possibility of exposing consumers to pathogenic B. cereus, this study develops a rapid and sensitive assay that utilizes immunoliposomal nanovesicles (IMLNs) and immunomagnetic beads (IMBs). In this work, fluorescent dyes (sulforhodamine B)-loaded IMLNs were employed to increase the detection signal; anti-B. cereus antibody-conjugated IMBs were applied to capture B. cereus in samples. Hence in this assay, a sandwich complex was formed as "IMBs-B. cereus-IMLNs". The optimal IMLNs had a diameter of 300 nm with a conjugated antibody molar percentage (mol%) of 0.25 mol%. The limit of detection (LOD) of this developed assay reaches 10 CFU/mL of B. cereus with the false negative value as zero in 20 parallel assays in milk samples. To evaluate the specificity of this assay, nine Gram positive and negative bacteria were tested and found to cause no significant interference problems. In conclusion, this study elucidates the feasibility of using a novel IMB/IMLN assay for detecting B. cereus and its LOD without pre-enrichment could amount to 10 CFU/mL within 4 h
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