We present direct measurements of the spatiotemporal electric field of an ultrashort Bessel-X pulse generated using a conical lens (axicon). These measurements were made using the linear-optical interferometric technique SEA TADPOLE, which has micrometer spatial resolution and femtosecond temporal resolution. From our measurements, both the superluminal velocity of the Bessel pulse and the propagation invariance of the central spot are apparent. We verified our measurements with simulations.

The theory of fluorescence correlation spectroscopy is reexamined with the aim of separating the contribution of rotational diffusion. Under constant excitation, fluorescence correlation experiments are characterized by three polarizations: one of the incident beam and two of the two photon detectors. A set of experiments of different polarizations is proposed for study. From the results of the experiments the isotropic factor of the fluorescence intensity correlation functions can be determined, which is independent of the rotational motion of the sample molecule. This function can be used to represent each fluorescence intensity correlation function as the product of the isotropic and the rotational factors. The theory is illustrated by an experiment in which rotational diffusion of porcine pancreatic lipase labeled with Texas Red was observed Texas Red is a label that allows precise fluorescence correlation experiments even in the nanosecond time range.

We measure the spatiotemporal field of ultrashort pulses with complex spatiotemporal profiles using the linear-optical, interferometric pulse-measurement technique SEA TADPOLE. Accelerating and decelerating ultrashort, localized, nonspreading Bessel-X wavepackets were generated from a approximately 27 fs duration Ti:Sapphire oscillator pulse using a combination of an axicon and a convex or concave lens. The wavefields are measured with approximately 5 microm spatial and approximately 15 fs temporal resolutions. Our experimental results are in good agreement with theoretical calculations and numerical simulations.

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A fluorescence correlation experiment for measurement of rotational diffusion in the nanosecond time scale is described. Using this method, the rotational diffusion coefficient of bovine carbonic anhydrase B labelled with tetramethylrhodamine isothiocyanate was estimated to be Dr = (1.14 +/- 0.15) X 10(7) s-1 at 22 degrees C. The experiment is based on a cw argon ion laser, a microfluorometer with local solution flow inside the sample cell, and two photon detectors. The fluorescence intensity autocorrelation function in the nanosecond time range is computed with the help of a time-to-amplitude converter and a multichannel pulse-amplitude analyser.