Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours.
Most super-resolution microscopy techniques depend on steps that can contribute to the formation of image artefacts, leading to misinterpretation of biological information. We present NanoJ-SQUIRREL, an ImageJ-based analytical approach that provides quantitative assessment of super-resolution image quality, capable of guiding researchers in optimising imaging parameters. By comparing diffraction-limited images and super-resolution equivalents of the same acquisition volume, this approach generates a quality score and quantitative map of super-resolution defects.
The cell wall of Staphylococcus aureus is characterized by an extremely high degree of cross-linking within its peptidoglycan (PGN). Penicillin-binding protein 4 (PBP4) is required for the synthesis of this highly cross-linked peptidoglycan. We found that wall teichoic acids, glycopolymers attached to the peptidoglycan and important for virulence in Gram-positive bacteria, act as temporal and spatial regulators of PGN metabolism, controlling the level of cross-linking by regulating PBP4 localization. PBP4 normally localizes at the division septum, but in the absence of wall teichoic acids synthesis, it becomes dispersed throughout the entire cell membrane and is unable to function normally. As a consequence, the peptidoglycan of TagO null mutants, impaired in wall teichoic acid biosynthesis, has a decreased degree of cross-linking, which renders it more susceptible to the action of lysozyme, an enzyme produced by different host organisms as an initial defense against bacterial infection.
Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples to benchmark and optimize the quality of microscopes, labels and imaging conditions. Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:
Staphylococcus aureus is an aggressive pathogen and a model organism to study cell division in sequential orthogonal planes in spherical bacteria. However, the small size of staphylococcal cells has impaired analysis of changes in morphology during the cell cycle. Here we use super-resolution microscopy and determine that S. aureus cells are not spherical throughout the cell cycle, but elongate during specific time windows, through peptidoglycan synthesis and remodelling. Both peptidoglycan hydrolysis and turgor pressure are required during division for reshaping the flat division septum into a curved surface. In this process, the septum generates less than one hemisphere of each daughter cell, a trait we show is common to other cocci. Therefore, cell surface scars of previous divisions do not divide the cells in quadrants, generating asymmetry in the daughter cells. Our results introduce a need to reassess the models for division plane selection in cocci.
Rising drug resistance is limiting treatment options
for infections
by methicillin-resistant Staphylococcus aureus (MRSA).
Herein we provide new evidence that wall teichoic acid (WTA) biogenesis
is a remarkable antibacterial target with the capacity to destabilize
the cooperative action of penicillin-binding proteins (PBPs) that
underlie β-lactam resistance in MRSA. Deletion of gene tarO, encoding the first step of WTA synthesis, resulted
in the restoration of sensitivity of MRSA to a unique profile of β-lactam
antibiotics with a known selectivity for penicillin binding protein
2 (PBP2). Of these, cefuroxime was used as a probe to screen for previously
approved drugs with a cryptic capacity to potentiate its activity
against MRSA. Ticlopidine, the antiplatelet drug Ticlid, strongly
potentiated cefuroxime, and this synergy was abolished in strains
lacking tarO. The combination was also effective
in a Galleria mellonella model of infection. Using
both genetic and biochemical strategies, we determined the molecular
target of ticlopidine as the N-acetylglucosamine-1-phosphate
transferase encoded in gene tarO and provide evidence
that WTA biogenesis represents an Achilles heel supporting the cooperative
function of PBP2 and PBP4 in creating highly cross-linked muropeptides
in the peptidoglycan of S. aureus. This approach
represents a new paradigm to tackle MRSA infection.
HighlightsSRRF is a purely analytical super-resolution microscopy approach available as an open-source easy-to-use plugin for ImageJ.SRRF is compatible with any fluorophore, including conventional fluorescent proteins such as GFP.SRRF can be used to retrieve super-resolution information from most common fluorescence microscopes.
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