RESUMENLa falla en la regeneración de las neuronas del sistema nervioso central (SNC) en vertebrados superiores es un problema que no se ha resuelto completamente, esto limita la rehabilitación de muchas conductas motoras después de una lesión en la médula espinal. En la regeneración neuronal intervienen múltiples factores y de estos, los que inducen el crecimiento neurítico se han estudiado para intentar favorecer la extensión y la reconexión de las neuronas lesionadas con sus blancos. La regeneración del SNC de sanguijuelas se ha estudiado intensamente porque permite abordar el problema a diferentes niveles con distintas técnicas, en este trabajo se obtuvo el espectro de absorción óptico, con espectroscopía fotoacústica (EFA), del SNC y de tejido de la sanguijuela Haementeria officinalis, con el objetivo de conocer las longitudes de onda óptimas para la irradiación posterior de células del SNC y de tejido H. officinalis. Los resultados de este estudio muestran que el SNC de estos organismos absorbe en la region comprendida de 300 nm a 500 nm, y las muestras de tejido tienen un máximo de absorción óptico proximo a 300 nm, además se observaron diferencias evidentes entre los espectros de absorción ópticos del SNC con lesión y el control (sin lesión).
The trachea serves a variety of functions during breathing and swallowing. In cases requiring a partial resection, the treatment may involve anastomosis of the healthy ends of the trachea. The tension in the anastomosis should not exceed 1,000 g. Traditional methods for estimating tension in the trachea are very invasive. The aim of this work is to develop a noninvasive optical system devoted to estimate the tension of the trachea in vivo. An optical system was designed using a light source and a photosensor. To determine the most suitable wavelength for the light source, a photoacoustic spectroscopy study was performed. To test the system, a cadaveric pig trachea was mounted on a universal testing machine, and it was subjected to three tensile tests of up to 30 mm elongation. The optical response was measured at 0, 10, 20, and 30 mm elongation. An exponential response was observed so that the optical voltage-response curves were adjusted, and three exponential equations were derived to correlate the voltage with the optical response. We can conclude that the proposed optical system is able to noninvasively estimate the tension of a cadaveric porcine trachea.
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