<p><strong>Background:</strong> The main problem in grapevine cultivation is root-attachment nematodes cause serious yield problems in most crops worldwide. Through their different infection mechanisms these nematodes synthesize and secrete a mixture of protein-based effectors that they use to penetrate the root, migrate and develop into giant root feeding cells in host plants. The use of new molecular tools such as MALDI TOF/TOF (Matrix-Assisted Laser Desorption/Ionization - Time-Of-Flight) mass spectrometry and PCR (Polymerase Chain Reaction) technique have allowed us to know these proteins and genes in different microorganisms. <strong>Objective:</strong> To characterize the root-knot nematode <em>Meloidogyne javanica</em> by sequencing the 18S rRNA gene from infected root samples and the effector proteins of juvenile (J2) and adult (J4) stage of <em>M. javanica</em> by MALDI TOF/TOF shotgun proteomics dual mass spectrometry. <strong>Methodology:</strong> Infected roots of grapevine crop were collected to extract fresh galls and J2 of <em>M. javanica</em>, then inoculated on tomato plants. J4 of <em>M. javanica</em> were used for genomic DNA extraction and sequencing at the 18S rRNA gene level. The J2 and J4 stages of <em>M. javanica </em>were disinfected with sodium hypochlorite (0.5%) and sterile distilled water for protein extraction and analysis with MALDI-TOF/TOF. Finally, the sequences obtained were processed with ProteinPilot™ and Protein BLAST software for the identification of effector proteins of <em>M. javanica. </em><strong>Results:</strong> The nematode <em>M. javanica</em> was molecularly identified by PCR amplification of the 18S rDNA gene <em>M. javanica</em> with an identity percentage of 98% from infected root samples and by MALDI TOF/TOF mass spectrometry, effector protein sequences were identified such as: Beta-1,4-endoglucan and polygalaturonase, identified from J2, and expansin B2, CLAVATA3/ESR, Pectate lyase and Chorismato mutase from J4, involved in the different infection processes. In addition, we were able to identify 49 nematode non-effector proteins in both stages related to conserved biological development.<strong> Implications:</strong> The results indicate the existence of effector proteins related to root gill formation. <strong>Conclusions: </strong>This study confirms that dual mass spectrometry methodology provides in a rapid and reproducible way a proteomic profile that the galls nematode synthesizes to infect root cells and that can be used in other types of pathogens.</p>
Especies de arañas como agentes de control biológico natural de la "cigarrita marrón" (Tagosodes orizicolus Muir) en el cultivo de arroz en el valle de TumbesSpider species as natural biological control agents of the "brown planthopper" (Tagosodes orizicolus Muir) in rice cultivation in the Tumbes valley
Las poblaciones de Tagosodes orizicolus Muir, insecto plaga clave del cultivo de arroz en el valle de Tumbes, son reguladas por enemigos naturales predadores y parasitoides. El objetivo del estudio fue identificar especies de parasitoides. Durante noviembre 2018 a junio 2019, se recolectaron semanalmente hojas de arroz conteniendo huevos; adultos y ninfas recolectadas con red entomológica y llevadas al Museo de Entomología de la Universidad Nacional de Tumbes, en las hojas se realizaron disecciones, se retiró la parte conteniendo los huevos, se depositaron en tubos de ensayo para esperar la emergencia de cigarritas o parasitoides para su identificación y preservación. Los adultos y ninfas fueron revisados para detectar presencia de ectoparasitoides. Se utilizaron las claves para especies de himenópteros neotropicales y subtropicales de Huber y Pinto. Se recuperaron dos especies, una perteneciente al género Anagrus (Mymaridae) y la otra al género Paracentrobia (Trichogrammatidae). El mayor porcentaje de parasitismo fue de 16,7% al 47,7% para Anagrus y 2,1% al 39,3% para Paracentrobia. No se recuperaron parasitoides de los otros estados de desarrollo. Se concluye que existen especies de parasitoides de huevos en el valle de Tumbes y para su multiplicación y protección natural hay que brindarles corredores biológicos.
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