Objetivo: Compreender se o local de coleta (com dentes ou desdentado) influencia na frequência de bactérias na cavidade oral. Foi realizado como um estudo observacional, prospectivo e transversal. Métodos: Amostras clínicas das superfícies orais dos dentes e/ou mucosa jugal foram coletadas na cavidade oral de 37 pacientes submetidos à cirurgia cardíaca eletiva no período pré-operatório de maio a julho de 2019. As amostras clínicas coletadas foram submetidas à identificação de colônias e testes antimicrobianos de sensibilidade. Resultados: Observou-se que independente do local de coleta ser dentado ou desdentado, o perfil microbiano, variáveis sociodemográficas, comorbidades e fatores de risco não influenciam estatisticamente na escolha do local de coleta. Conclusões: Não houve diferença estatística entre as cepas encontradas nos locais de coleta. O resultado encontrado é relevante para outros pesquisadores que trabalharão com coletas de cavidade oral, pois o local de coleta escolhido não influenciará na frequência de cepas encontradas.
Introduction. Leishmaniasis is among the 22 most neglected diseases worldwide. Few information is related about the evidence of neurological involvement that leishmaniasis can cause due to the ulcerations formed in the skin along short and long term. However, some authors have cited that leishmaniasis can cause inflammation in the central nervous system, which can trigger neuropsychomotor processes such as anxiety. Objective. The present study focuses on the evaluation of the effects of total Leishmania proteins on the central nervous system in the Zebrafish model. Method. Leishmania was initially cultured to extract total proteins by Sonication and Bradford methods. After total protein extraction, the zebrafish were inoculated to observe the symptomatology caused by protein load, and after seven days of inoculation, we performed the behavioral tests (Open Field and Bright & Dark). Results. The fish treated with Leishmania protein showed several cutaneous alterations, as well as behavioral changes. The open field behavioral tests showed a high number of crosses compared to the control group. In the light and dark test, the zebra fish had a longer stay in the light field, and an anxiolytic action was observed in the animals that received the protein administration. Conclusion. The total Leishmania proteins may have an anxiolytic action in the zebrafish model.
Objetivo: Verificar se houve relação entre a ocorrência de cepas bacterianas multirresistentes e o tempo de internação no pré-operatório. Métodos: Amostras clínicas das superfícies orais dos dentes e / ou mucosa jugal foram coletadas na cavidade oral de 37 pacientes submetidos à cirurgia cardíaca eletiva no período pré-operatório de maio a julho de 2019. As amostras clínicas coletadas foram submetidas à identificação de colônias e testes de sensibilidade antimicrobiana. Resultados: Observamos que os pacientes que permaneceram por mais de 60 dias naquele hospital tiveram 17 vezes mais chance de desenvolver cepas multirresistentes (Multi-Rs) do que os que não permaneceram. Conclusões: Percebemos que quanto mais tempo o paciente permanece internado, maiores são as chances de cepas bacterianas Multi-Rs. Portanto, é importante tentar reduzir o tempo de internação hospitalar para que não haja aumento na ocorrência de cepas multirresistentes nesses pacientes.Palavras-chave: Biofilmes. Bactérias; Saúde Oral; Infecção; Resistência Bacteriana a Drogas. ResumoObjective: Verify whether there was a relationship between the occurrence of multidrug-resistant bacterial strains and the length of stay in the preoperative period. Methods: Clinical samples of the oral surfaces of the teeth and/or cheek mucosa were collected in the oral cavity of 37 patients who underwent elective cardiac surgery in the preoperative period from May to July 2019. The clinical samples collected were subjected to identification of colonies and antimicrobial sensitivity tests. Results: We observed that the patients who stayed for more than 60 days in that hospital had 17 times more likely to develop multi-resistant strains (Multi-Rs) than those that have not remained. Conclusions: We realized that the longer the patient stays in the hospital, the greater the chances of bacterial strains Multi-Rs. Therefore, it is important to try to reduce the length of hospital stay so that there is no increase in the occurrence of multiresistant strains in these patients
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