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This paper proposes a new fiber optic sensor based on Regenerated Fiber Bragg Gratings specially designed to be embedded in concrete structures to monitor temperatures during fire events. A practical test was carried out on a 5.8m long beam subjected to the ISO-834 fire curve for 77 minutes under the typical loads borne by beams in conventional structures. Nine optical sensors were installed at the mid-span section of the beam and were submitted directly to flames and high temperature gradients (of the order of 200ºC/min) that make them measure maximum temperatures of 953º C. The temperatures recorded by the new sensors were compared with those obtained from electrical sensors (thermocouples) and a numerical model, with which they showed a good fit, except in those places in which concrete spalling caused distortions in the results and/or failure of the sensors. The paper thus demonstrates the viability of optical technologies in monitoring reinforced concrete during fires and analyzes sensor behavior to point out areas in which additional research is required.

Queuosine (Q) is a conserved hypermodification of the wobble base of tRNA containing GUN anticodons but the physiological consequences of Q deficiency are poorly understood in bacteria. This work combines transcriptomic, proteomic and physiological studies to characterize a Q-deficient Escherichia coli K12 MG1655 mutant. The absence of Q led to increased resistance to nickel and cobalt, and to increased sensitivity to cadmium, compared to the wild-type (WT) strain. Transcriptomic analysis of the WT and Q-deficient strains, grown in the presence and absence of nickel, revealed that the nickel transporter genes (nikABCDE) are down-regulated in the Q– mutant, even when nickel is not added. This mutant is therefore primed to resist to high nickel levels. Downstream analysis of the transcriptomic data suggested that the absence of Q triggers an atypical oxidative stress response, confirmed by the detection of slightly elevated ROS levels in the mutant, increased sensitivity to hydrogen peroxide and paraquat, and a subtle growth phenotype in a strain prone to accumulation of ROS.

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This paper describes an experimental study on patch-repaired reinforced concrete columns subjected to axial loads until failure. The behaviour of four series of columns repaired with Class R3 and R4 cement-based mortar was analysed both with and without a bonding agent. The results obtained were compared with control series of undamaged and damaged but unrepaired columns to determine the effectiveness of the repairs and the subsequent improvement in the behaviour of the columns. The results of this study were compared with those of previous work by the authors on the analysis of all-four-side repairs and one-side repairs. The conclusion reached was that patch repairs are highly effective as compared to the other techniques studied and that using Class R3 mortar achieves better results on damaged columns made with low-quality concrete. It was also concluded that applying a bonding agent does not improve the results, although this element was found to be necessary to prevent the premature detachment of the repair when there is a substantial difference between the stiffness of the column concrete and that of the mortar used in the repairs.