The aim of this study was to analyze the development of white blood cells in blood of neonatal piglets immediately after birth and colostrum intake. Together 8 newborn piglets (Large white) were included in this study. Piglets were during whole experiment under his mother and the colostrum intake was ad libitum. Blood samples (1.0 mL) were collected before colostrum intake (0 hour) and on 3 rd , 6 th and 12 th hour after first colostrum intake. Heparinized blood samples were used for total white blood cells, lymphocytes, mid-sized cells and granulocytes determinations. Blood samples were analyzed using haematological analyser Abacus Junior Vet (Diatron, Austria). The results were statistically analyzed by a one-way ANOVA, the differences in average means of blood cells between different sampling times were tested with T-test. The content of white blood cells (P<0.01), lymphocytes (P<0.001), mid-sized cells (P<0.05) as well as granulocytes (P<0.05) changed statistically during the first 12 hours of piglets life. Only in the samples collected on 6 th and 12 th hour of piglets life, the white blood cells (11.68 resp. 10.82 G*l-1) and lymphocytes (8.13 resp. 9.63 G*l-1) reached the lower physiological range for pigs. Other white blood cells indices of neonatal piglets did not reach the lower limit of the reference range. However in all white blood cells indices, we detected very high differences between minimal and maximal values. It points out, that some newborn piglets had very low, whereas some newborn piglets had very high content of white blood indices and it suggest, that some piglets of the litter had better protection, whereas another piglets not.
We would like to thank all colleagues who participated in its realization.
The reproductive form of Haemophilic infection caused by a microorganism Histophilus somni (Haemophilus somnus) is in our conditions practically unknown and undiscovered disease of cow reproductive organs. The aim of this work was based on experimental work to provide a comprehensive view on the pathohistological and submicroscopic changes in the uterus of 60 cows, serological positive, crossbreeds Slovenské strakaté x Holstein, aged 3.5 -4.5 years, serological positive, which were up to 90 days after the parturition sterile. After the hormonal control of the oestrus cycle (PGF2α-Oestrophan SPOFA) the cows were inseminated and the non-gravid cows later killed. The reproductive organs of killed animals were pathologically -anatomically dissected with following histological and sub-microscopic study of uterus and cervix tissue. At the beginning of the experiment no clinical changes of the reproductive organs of group 1 animals with the titer of antibodies 1:25-100 were diagnosed and in group 3 with the titer 1:25. At an examination before killing were examined endometritis (EM) of grade II. and vaginitis with vestibulitis in these groups. At the rest of animals appeared in every level of the titer of antibodies of EM II. and EM III. EM I. were not clinically diagnosed. Dissecting examination determined EM catarrhalis and cervicis catarrhalis in all groups. The histological examination pointed to EM catarrhalis chronica, EM interstitialis chronica, EM fibrousis and cervicis catarrhalis chronica. The leucocytes gained from a uterus lavage were in a comparison with stroma leucocytes mostly polynuclear (neutrophils) and in smaller amount small forms of lymfocytes. At long-term sterility of cows, because of possible H. somnus infection, we regard as priority the serodiagnostics at which is taken into consideration any level of the titer of antibodies.
Licensed under a Creative Commons Attribution 4.0 International License The aim of this work was to describe microscopic and submicroscopic changes in uterine tube of 40 sows in the estral cycle. Samples of the uterine tube were obtained for histological studies by vivisection from three sections of uterine tube. Samples were fixed for light microscopy (LM) in formaldehyde and in glutaraldehyde paraformaldehyde for scanning (SEM) and transmission (TEM) electron microscopy. They were subsequently processed in the usual manner in the LM and electron microscopic studies laboratories. We did not detect progressive changes in the length of the uterine tube. Unlike the sows' weight (2.57 ±1.12 g or 2.26 ±0.96 g), the length of the uterine tube was virtually unchanged depending on the stage of the cycle (30.2 ±2.51 cm in FF or 30.1 ±2.39 cm in LF). The largest relative volume of the epithelial layer was at the follicular stage of the cycle along the entire uterine tube. The difference varied from 4.99%-isthmus to 13.62% infundibulum between each part. Significant changes were seen between the ciliary and secretory cells during the estral cycle in the various parts of the uterine tube. Ciliary cells dominated throughout the cycle in infindibulum and ampulla, whereas secretory cells in isthmus. Their changes and differentiations are the manifestations of hormonal changes that direct the estral cycle. Submicroscopic changes of cells in the estral cycle have also been described.
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