To give the empirical space for development of molecular biotechnologies in regenerative medicine the article presents a systematic review of new data for the last 5 years, dedicated to chondrocyte receptor apparatus, connected to physiological and reparative regeneration in articular cartilage. The main attention is given to the receptors of chondrocytes involved in synthesis and degradation extracellular matrix, differentiation and apoptosis of chondrocytes.
Цель исследования -изучить морфологические характеристики суставного хряща тибиофемораль-ного сустава крысы с помощью метода многократного высокоточного сошлифовывания при моделирова-нии экспериментального остеоартроза.Материал и методы. Экспериментальное исследование проводилось на 16 половозрелых белых крысах-самцах линии Wistar. Моделирование остеоартроза проводилось путем введения в тибиофемо-ральные суставы 10 крыс суспензии стерильного медицинского талька с физиологическим раствором NaCl в соотношении 1:5. Животных выводили из эксперимента спустя 6 и 12 недель с помощью передозировки препарата «Bioveta» (Чехия) в дозе 200 мг/кг массы тела. При создании 3D-реконструкций была исполь-зована технология высокоточного послойного сошлифовывания в сочетании с цифровой съемкой шлифов и программное обеспечение для создания виртуальной модели объекта-оригинала. Количественное срав-нительное исследование было проведено с помощью программы «ImageJ».Результаты. Получены 3D-реконструкции суставов, лишенные пространственных и цветовых ис-кажений и позволяющие получать как правильные анатомические сечения в заданных плоскостях, так и нормали к поверхностям для точных линейных измерений. Описаны количественные показатели, харак-теризующие структуру суставного хряща в норме и при снижении лубрикативных свойств синовиальной жидкости. К 12-й неделе эксперимента толщина суставного хряща уменьшалась, в то время как фактор поверхности незначительно увеличился, что свидетельствовало о частичном повреждении суставной по-верхности. Полученные RGB-профили оцифрованных изображений суставного хряща тибиофеморально-го сустава крысы на 3D-реконструкциях позволили выявить новые ранние признаки дегенерации сустав-ного хряща при экспериментальном воспроизведении остеоартроза. Morphology of Tibiofemoral Joint in Rats with Experimental Osteoarthritis: 3D-reconstruction, Based on the Technology of High-Precision GrindingThe aim was to study morphological characteristics of rats' tibiofemoral joint articular cartilage using the method of multiple high-precision grinding in the simulation of experimental osteoarthritis.Materials and methods. An experimental study was carried out on 16 mature white male Wistar rats. Modeling of osteoarthritis was carried out by inserting into tibiofemoral joints of 10 rats the suspension of sterile medical talc with saline solution in the ratio 1: 5. The animals were withdrawn from the experiment after 6 and 12 weeks by an overdose of the drug "Bioveta" (Czech Republic) in a dose of 200 mg / kg of body weight. The technology of high-precision layer-by-layer sanding in combination with digital survey of thin sections and software for creating a virtual model of the original object were used in creating 3D-reconstructions. A quantitative comparative study was conducted using the "ImageJ" program.Results. 3D-reconstruction of joints devoid of spatial and color distortions were obtained, which allow to obtain both correct anatomical sections in given planes, and normals to surfaces for exact linear measurements. Qua...
Upon cartilage damage its natural recovery takes a long time. This is due to several reasons: the absence of blood vessels and low metabolic activity. In this regard, it is relevant to study the mechanism of operation of all components involved in the remodeling of cartilage tissue. For this purpose, protein insulin-like growth factor 1 (IGF-1) was taken as an object of research, since one of its main functions is regulation of cell proliferation, which is closely related to the process of cartilage tissue repair. It is known that in recent years, scientific studies have been conducted on the effect of IGF-1 on the remodeling of cartilage tissues, but in combination with other substances that stimulate more active cell regeneration. This review presents the features of the functional properties and protein structure and its main influence on chondrocyte proliferation for further development of accelerated and effective methods of IGF-1 action on cell growth and repair. The phylogenetic analysis of IGF-1 showed the most related organism for human IGF-1 and structural differences of its protein from human, which are inextricably linked with the functional characteristics of each of the organisms. The results of phylogenetic analysis in the future will identify the object for laboratory research in this question and the search for optimal ways to accelerate the process of remodeling of cartilage joints.
The article presents the results of study metaldependent proteins comprised in Artemisia annua proteome in the intrusion territories adjacent to the artificial agrobiocenosis in Volgograd region. Using mass spectrometry we detected more than 2200 proteins, including 134 metaldependent ones. One hundred twenty one of them had a dependence on zinc, 9 proteins depended on copper, and 4 proteins were manganese-dependent. We found no any nickelor cobalt-dependent proteins. Zinc-dependent proteins mainly worked in gene expression and energy metabolism regulation. Copper-dependent proteins involved in enzymatic reactions. Manganese-dependent proteins perform functions of DNA repair and enzymatic hydrolysis. According to these results, zinc-dependent proteins are the most prevalent from metaldependent proteins of Artemisia annua in the intrusion territories adjacent to the artificial agrobiocenosis of Volgograd region. Further study can stimulate the development of point control methods to reduce Artemisia population and its effect from the technogenic intrusion zone to adjacent agrobiocenosis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.