In this work, we present the development of a point-of-care platform for the serologic diagnosis of infectious diseases. The complete system consists of magnetic particles with immobilized antigens, disposable electrochemical cells, hardware and software. The main purpose of this paper is to present the last two components. The platform is powered by a rechargeable battery and can be controlled using mobile devices, allowing point-of-care diagnosis of diseases. The platform was successfully tested for the diagnosis of foot-and-mouth disease, human and bovine brucellosis, and Chagas disease.
The development of an inmunosensor for the point-of-care detection of the foot-and-mouth cattle disease is presented. The detector is based on an ELISA method with electrochemical detection. A non-structural protein, 3ABC, is used to selectively detect antibodies is used to selectively detect anti-3ABC antibodies produced after infection. The biological test is performed onto a screen printed electrodes. A dedicated small, portable potentiostat is employed for the control of the sensors, as well as data acquisition, processing, and storage.
h i g h l i g h t s• Magnetite-gold mesoparticles were prepared with controlled morphology by adjusting the concentration of reactants and surfactants used in the synthesis.• A structural study was carried out by using FIB/SEM technology which revealed that the inner structured of the particles is composed by iron oxide nanoparticles and gold crystals.• A tridimensional reconstruction was created from SEM images of sliced mesoparticles by focused ion beam.
t r a c tComposite magnetite-gold mesoscopic particles were prepared by deposition of Au onto magnetite nanoparticles. The surface morphology of the mesoparticles could be tuned by controlling the factors that affect the kinetics of the reactions and by adding surfactants that direct the growth of gold crystals. The internal structure of composite mesoparticles was analyzed by focused ion beam and scanning electron microscopy. A particle-mediated growth mechanism of formation was proposed.
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