Diagnosis of foot-and-mouth disease by electrochemical enzyme-linked immunoassay

Longinotti, Gloria; Ybarra, Gabriel; Lloret, Paulina; Moina, Carlos; Ciochinni, Andres; Serantes, Diego Rey; Malatto, Laura; Roberti, Mariano; Tropea, Salvador; Fraigi, Liliana

doi:10.1109/iembs.2010.5626230

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…Commercial carbon ink (Dupont BQ242), Ag ink (Dupont 5025) and Ag/AgCl ink (Dupont 5870) were employed. Others details of materials and manufacturing process are presented in [4]. …”

Section: Developmentmentioning

confidence: 99%

Smartphone controlled platform for point-of-care diagnosis of infectious diseases

Salomón

Tropea

Brengi

et al. 2014

2014 IEEE 9th IberoAmerican Congress on Sensors

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In this work, we present the development of a point-of-care platform for the serologic diagnosis of infectious diseases. The complete system consists of magnetic particles with immobilized antigens, disposable electrochemical cells, hardware and software. The main purpose of this paper is to present the last two components. The platform is powered by a rechargeable battery and can be controlled using mobile devices, allowing point-of-care diagnosis of diseases. The platform was successfully tested for the diagnosis of foot-and-mouth disease, human and bovine brucellosis, and Chagas disease.

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“…Commercial carbon ink (Dupont BQ242), Ag ink (Dupont 5025) and Ag/AgCl ink (Dupont 5870) were employed. Others details of materials and manufacturing process are presented in [4]. …”

Section: Developmentmentioning

confidence: 99%

Smartphone controlled platform for point-of-care diagnosis of infectious diseases

Salomón

Tropea

Brengi

et al. 2014

2014 IEEE 9th IberoAmerican Congress on Sensors

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“…A non-structural protein i.e. 3ABC of associated disease was detected through specific antibodies by utilization of a screen-printed electrode (Longinotti et al 2010 ). Hydatid disease is associated with Echinococcus granulosis.…”

Section: Development Of Nano-biosensing Devices For Detection Of Animmentioning

confidence: 99%

Nano-Biosensing Devices Detecting Biomarkers of Communicable and Non-communicable Diseases of Animals

Jain

Shakya

2021

Concepts and Strategies in Plant Sciences

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Diseases are either communicable or non-communicable. Communicable diseases or infectious diseases are caused by interactions between two living organisms whereas non-communicable diseases are either hereditary or due to the living environment of animals. Animals are very susceptible to various types of infections and pathogens may live outside or inside their body very conveniently. Epidemic diseases affect animal herd, livestock and the leading cause of animal mortality which may result in significant losses to the agricultural economy. Animal diseases are a matter of concern due to the direct effect on the economy as well as the possibility of pathogen transmission in humans. The accurate detection of animal diseases is an important factor to control diseases in livestock and in wild animals. Disease diagnosis in animals is very challenging and based on the variety of examinations and tests. In this context, nano-biosensors exhibit better disease detection capacity than conventional methods. Nano-biosensors show high sensitivity, stability, reproducibility with a lower limit of detection. This chapter emphasized the insight of communicable and non-communicable animal diseases and their detection through nano-biosensing devices. Some important animal diseases are discussed along with a description of developed nano-biosensors for them. Nano-biosensor functionalized with disease-related biomarkers performed better than any other conventional methods in various aspects.

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“…The important distinction of immunosensor assays is the method of signal transduction which may employ either an electrochemical or an optical transducer [ 20 ]. In electrochemical transducers, an electrochemical signal is measured, the magnitude of which changes following the formation of the Ag-Abs complex [ 21 , 22 ]. In the case of optical transducers, Ag-Abs quantification is based on the generation of light signals (color change or fluorescence) or changes in the optical properties of the environment [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma

Don¹,

Farafonova

Pokhil³

et al. 2016

Sensors

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In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected. The developed technique was found to provide high repeatability, intermediate precision and specificity. The difference between the analytical signals of RAF Ab samples and those of the placebo was up to 50.8%, whereas the difference between non-specific controls and the placebo was within 5%–6%. Thus, the piezoelectric immunosensor as well as ELISA has the potential to be used for detecting the effects of RAF Abs to IFNg on the antigen-antibody interaction, which might be the result of RAF’s ability to modify the affinity of IFNg to specific/related Abs.

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Diagnosis of foot-and-mouth disease by electrochemical enzyme-linked immunoassay

Cited by 5 publications

References 2 publications

Smartphone controlled platform for point-of-care diagnosis of infectious diseases

Smartphone controlled platform for point-of-care diagnosis of infectious diseases

Nano-Biosensing Devices Detecting Biomarkers of Communicable and Non-communicable Diseases of Animals

Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma

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