Summary Background Temporomandibular disorder (TMD) is a multifactorial condition involving environmental, psychological and genetic factors. Objective The aim of this case‐control study was to evaluate the influence of genetic polymorphisms in 5HTT and COMT on TMD and anxiety in adolescents. Methods TMD was diagnosed and classified according to the RDC/TMD criteria. For case group, the following TMD categories were used: myofascial pain, disc displacement, arthralgia and painful TMD (myofascial and arthralgia). Anxiety levels were assessed according to the State‐Trait Anxiety Inventory. Genomic DNA was extracted, and genetic polymorphisms were genotyped by TaqMan chemistry and endpoint analysis. Logistic multivariate regression was used to analyse the associations between TMD types and genotypes, anxiety level and genotypes, using an adjusted odds ratio (ORa; CI 95%) that considered the gender. Results In 5HTT, the rs1042173 was associated with painful TMD (arthralgia and myofascial pain) (ORc = 1.97; CI 95%: 1.02‐3.77; P = 0.04). Polymorphisms in COMT rs4818 were significantly associated with myofascial pain (ORc = 2.15; CI 95%: 1.08‐4.29; P = 0.02) and were borderline for painful TMD (ORc = 1.85; CI 95%: 0.97‐3.51; P = 0.06) and disc displacement (ORc = 2.42; CI 95%: 1.00‐5.87; P = 0.05). The rs6269 was borderline for myofascial pain (ORc = 1.82; CI 95%: 0.92‐3.59; P = 0.08) and disc displacement (ORc = 2.38; CI 95% 0.95‐5.97; P = 0.06) and also was associated with anxiety (ORa = 2.34; CI 95% 1.04‐5.25; P = 0.03). Conclusion Polymorphisms in 5HTT and COMT are associated with TMD in adolescents. Moreover, polymorphism in COMT is associated with anxiety in adolescents.

Introduction: Considering the large number of fixed functional appliances, choosing the best device for your patient is not an easy task. Objective: To describe the development of fixed functional appliances as well as our 20-year experience working with them. Methods: Fixed functional appliances are grouped into flexible, rigid and hybrid. They are different appliances, whose action is described here. Four clinical cases will be reported with a view to illustrating the different appliances. Conclusions: Rigid fixed functional appliances provide better skeletal results than flexible and hybrid ones. Flexible and hybrid appliances have similar effects to those produced by Class II elastics. They ultimately correct Class II with dentoalveolar changes. From a biomechanical standpoint, fixed functional appliances are more recommended to treat Class II in dolichofacial patients, in comparison to Class II elastics.

Facial and dental esthetics are the objectives of dental treatment, and, for this, it is necessary that specialties such as periodontics and orthodontics work together. The objective of this article is to report a clinical case with the solution of localized gingival recession after orthodontic treatment, using tunneled subepithelial connective tissue grafts with follow-up for 1 year. The patient underwent orthodontic treatment for 1 year. Prior to the start of treatment, a gingival recession of 5 mm was already present on tooth 31. One month after the completion of treatment, the patient was subjected to a tunneled subepithelial connective tissue graft, with the purpose of covering the exposed root. We observed the effectiveness of the procedure and patient satisfaction with the results obtained. The subepithelial connective tissue graft was successful in this case, and the collaboration of specialists is important to provide the best treatment for the patient.

Implantação de um software para controle da central de esterilização do curso de Odontologia da Universidade Positivo Revista da ABENO • 18(3):53-61, 2018 -DOI: http://dx.doi.org/10.30979/rev.abeno.v18i3.542 53 Implantação de um software para controle da central de esterilização do curso de Odontologia da Universidade Positivo Implantação de um software para controle da central de esterilização do curso de Odontologia da Universidade Positivo Revista da ABENO • 18(3):53-61, 2018 -DOI: http://dx.doi.org/10.30979/rev.abeno.v18i3.542 54

RESUMOIntrodução: A agenesia dentária (AD) é a ausência congênita de um ou mais dentes. Vários estudos vêm sugerindo o forte componente genético para essa condição. Objetivo: O presente estudo teve como objetivo avaliar se os polimorfismos genéticos nos genes que codificam os receptores de estrógeno (ESR1 e ESR2) estão associados à ocorrrência de AD isolada em uma amostra brasileira. Métodos: Radiografias panorâmicas de 142 pacientes ortodônticos foram avaliadas para determinar AD de dentes permanentes (excluindo terceiros molares). O DNA dos pacientes foi extraído das células da mucosa bucal contidas na saliva para avaliar polimorfismos genéticos em ESR1 (rs2234693 e rs9340799) e ESR2 (rs1256049 e rs4986938) por genotipagem usando a técnica de PCR em tempo real. Para análises estatísticas, associações entre as distribuições dos alelos e genótipos e a ocorrrência de AD foram avaliadas para cada polimorfismo genético, com um alfa estabelecido de 5%. Resultados: Treze pacientes tiveram pelo menos 1 dente congenitamente ausente. O número de dentes congenitamente ausentes variou de 1 a 11. Os polimorfismos genéticos rs2234693 e rs9340799 no ESR1 e rs1256049 no ESR2 não foram associados à AD (p > 0,05). Para o polimorfismo genético rs4986938 no ESR2, as distribuições dos genótipos e dos alelos foram estatisticamente diferentes entre os pacientes com e sem AD (p < 0,05). O genótipo CC e o alelo C estavam super-representados nos pacientes com AD. Conclusão: Houve associação entre o polimorfismo genético rs4986938 no ESR2 e a ocorrrência de AD. ABSTRACT Introduction:Tooth agenesis (TA) is the congenital absence of teeth. Several studies have proposed a strong genetic background for this condition. Aim: The present cross-sectional study aimed to evaluate whether genetic polymorphisms in the genes that code for estrogen receptors (ESR1 and ESR2) are associated with the presence of isolated TA in a Brazilian sample. Methods: Panoramic radiographs of 142 orthodontic patients were assessed to determine TA of permanent teeth (excluding third molars). DNA of patients was extracted from buccal cells from saliva to evaluate genetic polymorphisms in ESR1 (rs2234693 and rs9340799) and ESR2 (rs1256049 and rs4986938) by genotyping using the real-time PCR technique. For statistical analyses, associations between the distributions of the alleles and genotypes, and the ocurrence of TA were assessed for each genetic polymorphism, with an established alpha of 5%. Results: Thirteen patients had at least 1 congenital missing tooth. The number of congenitally missing teeth ranged from 1 to 11. The genetic polymorphisms rs2234693 and rs9340799 in ESR1 and rs1256049 in ESR2 were not associated with TA (p > 0.05). For the genetic polymorphism rs4986938 in ESR2, the genotype and allele distributions were significantly different between the patients with and without TA (p < 0.05). The CC genotype and the C allele were overrepresented in the TA patients. Conclusion: The genetic polymorphism rs4986938 in ESR2 was associated with the ocurrence of TA.

Zirconium dioxide implants, also called zirconia implants, have emerged on the market as a material option in rehabilitation with osseointegrated implants and their use has had an upward learning curve over the last few years. The aim of this study was to report a case in which an immediate single-body zirconia implant was used, associated with a subepitlhelial connective tissue graft, and immediate loading. The results showed totally adequate, from both a technical point of view and in terms of patient’s satisfaction. Clinical studies with long term follow-up are needed to further clarify the properties, advantages, and indications of zirconia implants

Periodontal disease is a multifactorial pathology that leads to the loss of tooth support structures, often compromising function and esthetics. For periodontal treatment to be successful, it is necessary to remove the pathogenic biofilm through scaling and root planning. Currently, the use of proteins derived from the enamel matrix can be associated with periodontal therapy, promoting a better result. The aim of this study was to report a case of periodontal treatment combining scaling and root planning, antibiotic therapy, and enamel matrix derivatives. In this case, it was observed that periodontal treatment associated with enamel matrix derivatives promoted good periodontal tissue healing, favoring the treatment result.

Inorganic bovine bone matrix (IBBM) is a biomaterial with proven osteoconductive functionalities. The objective of this study was to assess the in vivo bone regeneration functionalities of IBBM modified or not by an experimental MOE in sheep. MOE synthesis was performed by suspending nacre particles (0.05 g, diameters < 0.01 mm) in anhydrous acetic acid (pH 7, 5 mL, 25°C, 72 hours) using magnetic stirring. Polyethylene carriers (d= 5.0 mm, l= 10.0 mm, open ends) of negative control (sham) or experimental groups (IBBM or MOE-modified IBBM) were placed (n=3 conditions /animal; intramuscularly) adjacent to the lower spine of adult sheep (8 animals, » 45 Kg, 2 years old). Tissues were harvested (at 3 or 6 months) after implantation in preparation for histological (H), morphometrical (MM) and immunohistochemical analyses (IH; Wnt-3a, CD34, Vimentin and PREF-1). MM data were tested for normality and variance homogeneity using the Shapiro-Wilk and Levene tests, and Mann Whitney and Kruskal-Wallis, respectively. IM data were analyzed using two-way ANOVA and Tukey tests. Differences (p < 0.05) were observed between experimental groups (IBBM and IBBM+MOE at both 3 and 6 months) and controls (sham) for total area; Differences were not found for presence of remnant particles among experimental groups. The highest formation of bone was observed with IBBM+MOE (6-months). No differences (p > 0.05) were found on IM analysis (CD34, Vimentin, PREF-1, Wnt3a). Results indicated that experimental materials (IBBM+MOE) display promising functionalities. Additional studies are necessary to define biomaterials’ longitudinal effects and long-term biocompatibility properties.