Artículo de publicación ISIRoot hypoxia in fruit trees affects growth, vegetative
development, and reproductive development, which
is reflected in low productivity, poor fruit quality, and
premature decay of trees. Using Illumina Hiseq2000, we performed transcriptome analysis of roots from two different
rootstocks, ‘Mariana 2624’ and ‘Mazzard F12/1,’
which are tolerant and sensitive to hypoxia, respectively.
Transcriptomes from control and hypoxia-stressed plants
(6, 24, and 72 h) were compared, using Prunus persica
(L.) as reference genome. Hypoxic conditions altered the
transcription in both genotypes. There were a high number
of common differentially expressed genes (DEG) between
the two genotypes for each sampling time, but also exclusive
DEG for each genotype, with a few DEG that presented
opposite modes of regulations during the hypoxia
treatment. An important group of DEGs exclusively upregulated
in the tolerant genotype are associated to enzymes
of posttranslational protein modifications, such as leucinerich
repeat (LRR), kinases and ubiquitin-protein ligases,
regulation of transcription, and process of oxide reduction.
Singular enrichment analysis of gene ontology (GO), detected
at least 115 GOs involved in the response to root
hypoxia in the sensitive and/or tolerant genotypes. At least
25 GOs were identified as part of the baseline differences
between the genotypes, most GO were disturbed in the
sensitive genotype. The contribution from the baseline
gene expression to the differential response between the
Prunus genotypes is evidence that the resistant genotype
is already Bprepared^ for a hypoxia event. An example are
GO BP:0042221 of response to chemical stimulus;
BP:0006979 of response to oxidative stress; MF:0016209
of antioxidant activity; MF:0016684 of oxidoreductase activity,
acting on peroxide as acceptor; and MF:0004601 of
peroxidase activity, which were disturbed only in the sensitive
genotype, but not in the tolerant.FONDECYT (No. 1121117) and CEAF_R08I100
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.