Softening rate of the Chilean strawberry (Fragaria chiloensis) fruit reflects the expression of polygalacturonase and pectate lyase genes

Figueroa, Carlos R.; Pimentel, Paula; Gaete-Eastman, Carlos; Moya, Mario; Herrera, Raúl; Caligari, P. D. S.; Moya-León, Marı́a Alejandra

doi:10.1016/j.postharvbio.2008.01.018

Cited by 84 publications

(86 citation statements)

References 36 publications

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“…The structural features of two genes were different from plC, and the spatio-temporal expression profiles were not the same with plC. The expression pattern of FcPL1, cloned from F. chiloensis, was different to that of FaPL from F. ananassa (Figueroa et al 2008). Five PL subtype sequences (DQ076239, DQ076240, DQ076241, DQ076242, and DQ076243) were isolated from strawberry cultivar 'Elsanta'.…”

Section: Discussionmentioning

confidence: 96%

“…PlA and PlB, two PL genes cloned in strawberry fruits, demonstrated specific expression patterns during fruit softening (BenitezBurraco et al 2003). Comparing with F. ananassa, FcPL1 cloned from F. chiloensis showed a differential expression profile (Figueroa et al 2008). In addition, two PL genes cloned from ripe banana exhibited different expression patterns in the pulp and in the skin (Pua et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of pectate lyase in relation to softening in strawberry fruits

Zhou¹,

Li²,

Zhao³

2016

Can. J. Plant Sci.

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Strawberry fruits are perishable due to fruit softening occurring during storage and marketing, but the mechanism by which this occurs is not clear. In this study, the possible mechanism of softening is explored by comparative studies on the changes of fruit firmness, pectate lyase (PL) activities, and relative expression levels of PL genes between two strawberry cultivars. The results indicate that a method for the determination of PL activity in the strawberry fruit was established. The activity of PL was at a low level in the small green fruit to white fruit stage, and maintained at a high level in the ripening and softening stage. The PL gene was specifically expressed in fruit and mainly in the ripening stage. PL expressions were closely correlated with PL activities, and negatively related with the changes in flesh firmness. The full-length cDNA of PL genes from the two cultivars was cloned using the RACE method. Amino acid residues of C terminal sequences of the PL proteins from different species showed significant variations. Those results suggested that the differences in PL activity, gene sequence, and gene expression patterns may lead to the different roles of PL in different fruit textures of strawberries during ripening and softening.Key words: fruit ripening and softening, pectate lyase, strawberry.Résumé : La fraise est un fruit périssable parce que la baie ramollit pendant l'entreposage et la vente, mais on ne sait pas exactement quel mécanisme est à l'origine de ce phénomène. Les auteurs se sont efforcés de le préciser en comparant les changements qui surviennent au niveau de la fermeté du fruit, de l'activité de la pectate lyase (PL) et de l'expression relative des gènes PL chez deux cultivars de fraisier. Les résultats ont permis d'établir une méthode pour jauger l'activité de la PL chez la fraise. La PL est peu active dans les petites baies vertes, jusqu'au stade du fruit blanc, après quoi elle reste très active pendant le mûrissement et le ramollissement. Le gène PL s'exprime spécifiquement chez le fruit et surtout pendant le mûrissement. L'expression du gène PL est étroitement corrélée à l'activité de la PL et négativement reliée aux changements de fermeté de la chair. Les auteurs ont cloné l'ADNc complet des gènes PL des deux cultivars par la technique RACE. Les résidus des acides aminés de la séquence C finale des protéines PL varient significativement entre les deux variétés. Ces résultats donnent à penser que les écarts au niveau de l'activité de la PL, de la séquence génétique et de l'expression des gènes pourraient expliquer le rôle variable de la PL dans les diverses textures observées chez la fraise pendant le mûrissement et le ramollissement. [Traduit par la Rédaction] Mots-clés : fraise, pectate lyase, mûrissement, ramollissement du fruit.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Comparative analysis of pectate lyase in relation to softening in strawberry fruits

Zhou¹,

Li²,

Zhao³

2016

Can. J. Plant Sci.

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“…PG has been shown to be a key enzyme in the softening of soft fruit and its function is maintained during the entire softening process (Figueroa et al, 2008). While, with regard to hard fruit, PG is possibly involved in the initiation of fruit ripening instead of the initiation of softening and merely functions as a start-up enzyme (Redondo-Nevado et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of polygalacturonase in the fruit of strawberry cultivars

Zhou¹,

Li²,

Zhao³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The role of polygalacturonase (PG) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. Changes in PG activity and gene expression during development, ripening and softening were measured. The PG genes from each cultivar were cloned and analyzed, and were classified with other PG genes using phylogenetic analysis. In Toyonoka fruit, PG activity increased gradually, reaching a peak during the pink stage, and remained at this level during post-harvest softening. Changes in PG gene expression were consistent with PG activity in these softer fruits. In the firmer Sweet Charlie fruits, PG activity was detected during the initial development stage, reaching a peak at the white stage, thereafter decreasing gradually with ripening and remaining at this lower level throughout softening. Changes in PG gene expression and PG activity were not consistent in these fruit. For both Toyonoka and Sweet Charlie PG genes (FaTPG and FaSCPG, respectively), the open reading frame was 1218 bp, encoding 405 amino acids. Five different nucleotide sites were observed between the two sequences, leading to two amino acid sequence mutations. FaTPG, FaSCPG, and PG genes from the Fragaria vesca genome were classified into three clades using phylogenetic analysis. The clade containing PG genes involved in fruit

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“…It has been reported that color changes during ripening are not so pronounced in Chilean strawberry fruit (Figueroa et al, 2008(Figueroa et al, , 2010. However, there are cases, where the MeJA treatment accelerated the acquisition of red color during an in vitro ripening assay in F. chiloensis fruit (Concha et al, 2013).…”

Section: Effect Of Meja and Chitosan On Fruit Quality Parametersmentioning

confidence: 98%

Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of Fragaria chiloensis fruit

et al. 2016

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Softening rate of the Chilean strawberry (Fragaria chiloensis) fruit reflects the expression of polygalacturonase and pectate lyase genes

Cited by 84 publications

References 36 publications

Comparative analysis of pectate lyase in relation to softening in strawberry fruits

Comparative analysis of pectate lyase in relation to softening in strawberry fruits

Comparative analysis of polygalacturonase in the fruit of strawberry cultivars

Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of Fragaria chiloensis fruit

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