CDC6 is an essential gene of yeast Saccharomyces cerevisiae. Although DNA sequence of the gene is available for a long time, biochemical function of Cdc6 protein in the cell cycle remains unclear. Using the interaction trap experiment we were looking for proteins interacting specifically with Cdc6. Four gene products interacting with Cdc6 were detected. By sequence analysis we found that ECM11 codes for the protein involved in the cell wall synthesis, YNL201 codes for the protein of unknown function, probably involved in the carbon metabolism, YOR279 codes for protein of completely unknown function with no significant similarity with any known protein, and the interaction with Ty1 retrotransposition element was also found. The strongest interaction with Cdc6 bait measured as beta-galactosidase activity was observed with ECM11 and YNL201; YOR279 interacts slightly weaker. The weakest beta-galactosidase activity was obtained by Ty1A element. The strongest suppression of cdc6-1 mutation was observed by Ty1A element, the slight one with ECM11 and YNL201 but no suppression of thermosensitive mutation was detected for YOR279.
A sudden increase in the growth temperature of Methylophilw methylotrophus results in the synthesis of a number of unique proteins. The major heat-shock proteins have apparent molecular masses of 83, 78, 63, 60, 16 and 14 kDa. Other stress conditions elicit a similar response, although there are significant differences in the sets of proteins produced under the various conditions. Addition of methanol induces proteins identical in size to the heat-shock 83,78,63 and 14 kDa proteins and also induces unique 94,36 and 29 kDa species. Addition of ethanol induces proteins identical in size to the 78 and 20 kDa heat-shock proteins and the 94 and 36 kDa methanol-induced proteins and an apparently unique 13 kDa species. Simultaneous exposure to elevated temperature and either methanol or ethanol resulted in the synthesis of all of the proteins induced by the separate treatments. The stress-shock proteins are differentially located in cytoplasmic, periplasmic and membrane fractions.Heat shock; Methanol; Ethanol; Stress; (h4ethylophilu.s methylotrophus)
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