During the vegetation periods 1994 and 1995, net uptake of nitrate and ammonium by roots of adult spruce (Picea abies (L.) Karst) and beech (Fagus sylvatica L.) trees was studied at a field site exposed to high loads of N (' Ho$ glwald ', Germany). In addition, uptake experiments were carried out under controlled conditions with young spruce and beech trees grown at normal N supply.In the field, nitrate was not taken up by the roots of spruce trees in appreciable amounts. This was also true for beech except during September 1995. Apparently, beech trees was capable of taking up nitrate, but the environmental condition prevailing at the field site usually prevented net uptake. Net uptake of ammonium in both tree species showed a seasonal course, with maximum rates in mid summer. Rates of ammonium uptake by both species correlated with soil temperature at the field site.Laboratory experiments on the influence of root temperature on uptake of nitrate indicated that uptake rates at temperatures found in the field were low compared with the uptake capacity at optimum temperature. At temperatures of 10 and 15 mC, frequently found in the soil at the field site, net uptake of nitrate by spruce and beech amounted to c. 16 % and 11 %, respectively, of maximum uptake at 25 mC. By contrast, net uptake of ammonium at 10 mC reached 73 % and 31 % of the maximum uptake for spruce and beech trees, respectively. Independent of temperature, rates of nitrate uptake were considerably lower than those of ammonium. In young spruce and beech trees, net uptake of nitrate was significantly inhibited by ammonium at nitrate : ammonium ratios found in the soil solution at the forest site. Preincubation of roots of both species, with amino acids present in the phloem of adult trees at the field site, led to an increase in the amino acid pool in the roots. For spruce trees a correlation between inhibition of uptake of nitrate and enrichment of the roots with the amino compounds Glu, γ-amino butyric acid (Gaba), Gln, and Asn was observed. In beech trees, enrichment of Asp and Gln in the roots correlated with a decrease in net uptake of nitrate. The results of laboratory experiments on the effects of temperature, the nitrate to ammonium ratio in the nutrient solution, and amino acid enrichment in the roots are discussed with special emphasis on the patterns of net uptake of ammonium and nitrate observed in the field.Key words : Nitrate uptake, ammonium uptake, soil temperature, nitrate to ammonium ratio, amino acids. In forest ecosystems, nitrate and ammonium are the most abundant N compounds available to the roots * To whom correspondence should be addressed. E-mail : here!sun2.ruf.uni-freiburg.de of trees. Total amounts of nitrate and ammonium, and the ratio of nitrate to ammonia depend on quality and quantity of N input and on the balance of ammonification, nitrification, immobilization and denitrification processes in the soil (Haynes & Goh,
summary During the vegetation period 1994, the nitrogen (N) composition and contents of leaves, xylem sap and phloem exudates of twigs from a coniferous (Picea abies (L.) and a deciduous (Fagus sylvatica L.) tree species were analysed at a field site (Höglwald, Germany) exposed to high loads of N. In April, total soluble non‐protein N (TSNN) in the xylem sap of twigs from spruce and beech reached a maximum of about 7.0 and 15.0 μmol ml−1, respectively, probably owing to remobilization of stored N. After bud break, TSNN in the xylem sap of both tree species decreased rapidly, to a minimum of c. 2.0 μmol ml−1 in July. Subsequently, TSNN increased two‐fold in the xylem sap of beech until September, but remained constant in the xylem sap of spruce at the low level observed in July. In both tree species, amides were the predominant TSNN compounds transported in the xylem. In xylem sap of beech. Gin, Asn and Arg were most abundant, together comparing more than 60% of TSNN. Gin and Asp, but not Arg, prevailed in the xylem sap of spruce, together comparing more than 50 % of TSNN. In addition, a number of other proteinogenic and non‐proteinogenic amino acids, and also nitrate and ammonium, were detected in small amounts in the xylem saps of both tree species. Remobilization of stored N during growth of the new flush resulted in a 4.5‐fold decrease of TSNN in previous year's needles from 9.1 μmol g−l f. wt in April to 2.0 μmol g−1 f. wt in May. This decrease was mainly attributed to Arg, the prevailing amino compound in needles of spruce. Subsequently, a 5.5‐fold increase was observed in July. High TSNN contents remained constant until September, Within current year's needles TSNN remained relatively constant during the entire vegetation period. In leaves of beech, TSNN amounted to c. 5.6 μmol g−1 f, wt from April to July and increased to 9.5 μmol g−1 f. wt in September. In April and May the predominant amino compounds in leaves of beech were Asn and Glu, in September they were Arg and Gin. Concomitant with the increase in Arg content of the leaves, its content in the phloem also increased, suggesting a transport of Arg from the senescencing leaves to storage tissues. Nitrate was found neither in needles nor in leaves, whereas ammonium amounted to up to 25 % of TSNN in both needles and leaves. In phloem exudates of twigs from beech, TSNN amounted to c. 34.0 μmol g−1 f. wt in April and decreased 10‐fold until May, during growth of the new flush. Subsequently, until September a threefold increase in TSNN was observed. In phloem exudates of twigs from spruce, TSNN decreased from 13.0 μmol g−1 f. wt in April to 4.8 μmol g−1 f. wt in May, By September, TSNN contents were doubled. In April, Arg was the prevailing amino acid in phloem exudates of beech. Concomitant with the decline in total TSNN the Arg content decreased. In September a significant increase in the Arg content was observed. In phloem exudates of spruce, Gin and Arg were the predominant N compounds in April and May. In July and September a decrease of the Gin conte...
During the growing session of 1995, the total soluble non‐protein nitrogen (TSNN) composition and contents of mycorrhizal fine roots, xylem sap and phloem exudates of roots from a coniferous (Picea abies L.(Karst)) and a deciduous (Fagus sylvatica L.) tree species were analysed at a field site (‘Höglwald’, Germany) exposed to high loads of N. In April, TSNN in fine roots of spruce and beech trees amounted to 16 μmol N g−1 f. wt and 23·3 μmol N g−1 f. wt, respectively. It decreased to 9·2 μmol N g−1 f. wt and 18·1 μmol N g−1 f. wt, respectively, after bud break in June. The seasonal maximum of TSNN in fine roots of spruce was observed in July (32·7 μmol N g−1 f. wt) followed by a decline of c. 30% until the end of the growing season in September. TSNN in fine roots of beech trees showed a further decline between June and July, when its seasonal minimum was determined (15·6 μmol N g−1 f. wt), and increased to c. 29 μmol N g−1 f. wt until September. In spruce roots Gln and Arg were the most abundant TSNN compounds during the entire growing season. In roots of beech Asn played an important role alongside Gln and Arg, especially in April, when it was the most abundant TSNN compound. Other proteinogenic and non‐proteinogenic N compounds comprised c. 20–30% of TSNN. Nitrate made up <1%, and ammonium <7% of TSNN in the fine roots of both species. In April, TSNN in the xylem sap of roots of spruce and beech trees amounted to 3·4 and 8·6 μmol N ml−1, respectively. In roots of spruce trees xylem sap TSNN increased after bud break up to 12·7 μmol N ml−1 in July. At the end of the growing season TSNN had declined again to 3·9 μmol N ml−1. TSNN in the root xylem sap of beech trees decreased after bud break until July (2·4 μmol N ml−1 in July) followed by a slight increase until September (2·9 μmol N ml−1). Arg, Gln and Asp were the most abundant TSNN compounds in the xylem sap of spruce trees contributing together c. 90% to TSNN. The same TSNN compounds prevailed in the root xylem sap of beech trees in April and July, whereas in June and September Asp was replaced by Asn comprising 57% of TSNN in June. In addition to the N compounds mentioned above, a number of other proteinogenic and non‐proteinogenic amino compounds were found in root xylem sap of both species. In either species, nitrate and ammonium were present in small amounts, contributing <1% and <4% to TSNN, respectively. Apparently, inorganic N taken up by the mycorrhizal roots is mainly assimilated in root tissues or by the mycorrhiza and N uptake by the roots is largely adapted to the assimilatory capacity of this organ. In phloem exudates of spruce roots, TSNN amounted to 10·7 μmol N g−1 f. wt in April, increased in June to 23·4 μmol N g−1 f. wt and decreased again until September to a seasonal minimum of 4·8 μmol N g−1 f. wt. In contrast to spruce, TSNN content in phloem exudates of beech roots showed a seasonal maximum (c. 20 μmol N g−1 f. wt) in April with a subsequent decrease in June after bud break (c. 2 μmol N g−1 f. wt). A fourfold increase in July was foll...
High loads of nitrogen to spruce and beech forests can result in a complete inhibition of NO $ − uptake by the roots of the trees. This conclusion is based on (a) a comparison of a field site continuously exposed to high loads of N and a N-limited site, (b) the results of N fertilization of a N-limited field site, and (c) laboratory experiments under controlled environmental conditions. From fertilization experiments in the field it appears that NH % + uptake might become inhibited subsequent to an excessive uptake of NH % + . Apparently, the inhibition of NO $ − uptake by high loads of N to forests is a consequence of an accumulation of organic amino compounds in the roots originating from phloem transport from the shoot to the roots. These amino compounds seem to signal the N demand of the shoot to the roots. At present this function cannot be attributed to an individual organic amino compound in beech or spruce, but Gln is a likely candidate in both species among other compounds, e.g. Glu in spruce or Asp in beech trees. Direct inhibition of NO $ − uptake by NH % + can be excluded from the present studies. The mechanism(s) by which elevated levels of particular organic amino compounds interact with NO $ − uptake remains to be elucidated. This (these) mechanism(s) seem to affect NO $ − influx rather than NO $ − efflux. As a consequence of this (these) mechanism(s), spruce and beech trees can prevent, within a certain physiological window, N over-nutrition when the roots are exposed to excessive amounts of inorganic N. However, inhibition of NO $ − and NH % + uptake by the roots makes more N available for leaching into the ground water and, in addition, for soil microbial processes that result in the production and re-emission of volatile N compounds into the atmosphere.At the ' Ho$ glwald ' site, continuously exposed to high loads of N, 20 % of the N input from throughfall into the spruce and beech plots is re-emitted as NO and N # O. However, the NO to N # O ratio is highly dependent on the tree species, with a preference for NO in the spruce and a preference for N # O in the beech plot. Since at least part of the NO emitted from the soil will be converted inside the canopy in the presence of ozone to NO # that might then be absorbed by the leaves, the portion of the N in the throughfall that will be released from the forest by gaseous N emission is higher in the beech than in the spruce plot. Leaching of NO $ − into the ground water is high in the spruce, but minute in the beech plot. However, this positive effect of beech on ground water quality is achieved at the expense of an enhanced release of radiatively active N gases into the troposphere.Key words : Atmospheric pollution, forest ecosystem, nitrogen allocation, nitrogen fluxes, regulation, nitrogen oxides, ammonia. Both atmospheric and pedospheric sources of nitrogen are available to plants. The contribution of atmospheric N is considered to be low in remote * To whom correspondence should be addressed E-mail : here!sun2.ruf.uni-fre...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.