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Concentrations of total Aluminium (Al) and Iron (Fe) were determined in Lake Oreochromis mossambicus was along a mean sequence of intestine > yellow body fat > brain > gills > liver > heart > white body fat, while the mean sequence of total Al and Fe in Micropterus salmoides was: intestine > gills > liver > heart > brain > white body fat. From the levels of Al detected in the yellow body fat of the studied fish species Oreochromis mossambicus, we suggest that this phenomenon may be related to the feeding habits of this species. Furthermore, the intake of certain species of phytobenthos by Oreochromis mossambicus could have played a role in the bioaccumulation of Al in the food chain and the possible development of pansteatitis in predators at higher trophic levels.

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1. Pansteatitis (yellow fat disease) is ubiquitous in the free-ranging population of Oreochromis mossambicus from Loskop Reservoir (LR), South Africa. The disease is nutritionally mediated and associated with a diet high in polyunsaturated or rancid fats, frequently of fish origin. While piscivory has never been reported in dietary studies of O. mossambicus in their native range, their opportunistic and omnivorous feeding habits mean that piscivory cannot be ruled out as a cause of the disease. 2. The diet of O. mossambicus from LR (n = 91) was compared with a population from Flag Boshielo Reservoir (FBR; n = 81) located <100 km downstream, where no pansteatitis occurs. The stomach contents and stable isotope signatures (d 15 N and d 13 C) of fish and food sources were evaluated across four seasons. Isotope signatures were also compared over various time scales from historic samples and mortalities collected from LR. 3. There was no evidence of piscivorous feeding behaviour in fish from either location or from historic LR samples. The results of the stable isotope analysis in R mixing model and stomach contents analysis showed that the dinoflagellate, Ceratium hirundinella, was the dominant food source followed by zooplankton, detritus and Microcystis aeruginosa in LR. The diet of fish from FBR was less diverse than fish from LR and was dominated by sediment and detritus. 4. The distinguishing feature of the dietary comparison between reservoirs was the abundance of planktonic food items dominated by C. hirundinella in the diet of fish from LR. The lack of evidence for piscivory among O. mossambicus from LR suggests that the classic aetiology of pansteatitis does not apply. This highlights the need to further explore direct (environmental exposure to pollutants) and indirect (dietary exposure) links to pansteatitis. This study identified the major dietary constituents for O. mossambicus, which enables future research to focus on their nutritional and chemical composition.

a b s t r a c tThe eutrophic process potentially caused by a high urine and faecal load resulting from an unusually high hippopotamus (Hippopotamus amphibious) density in the Nhlanganzwane Dam, Kruger National Park, South Africa, triggered a chain of events characterised by an increase in the growth of primary producers (Microcystis aeruginosa). This increase in M. aeruginosa biomass was followed by biointoxication incidents in wild animals. In this study, we determine if a M. aeruginosa bloom with a total microcystin level of 23,718 mg l À1 have been responsible for mortalities of megaherbivores in the Nhlanganzwane Dam. We further use microcystin molecular markers derived from the mcy gene cluster to identify potentially toxigenic environmental Microcystis strains in the dam during the occurrence of animal intoxications. The estimated total microcystin-LR daily intake by an adult male white rhinoceros (Ceratotherium simum) from cyanobacterial-contaminated water of the dam during the toxic event was an order of magnitude higher (754.29 mg kg À1 bw) in comparison with the lowest observed adverse effecting level (LOAEL) value measured for pigs in a previous study by other authors. In this study the presence of toxic cyanobacterial strains was confirmed with the use of molecular markers that detected the presence of the mcy gene cluster responsible for the production of toxin by M. aeruginosa.

A declining crocodile population and fish mortalities attributed to pansteatitis, along with increasing blooms of Microcystis aeruginosa and Ceratium hirundinella, have led to serious concerns about water quality in Loskop Dam, on the Olifants River, South Africa. Major impacts include acid mine drainage and eutrophication associated with sewage effluent. However, the specific causes of pansteatitis remain elusive. In 2011 the water chemistry and limnology of Loskop Dam were studied to determine factors that may be influencing aquatic ecosystem health. Long-term monitoring data collected by the Department of Water Affairs were analysed for trends using a seasonal Mann-Kendall trend test, and were used to determine the trophic state of Loskop Dam using the Carlson index. Multiple sites were sampled which showed the reservoir was heterogeneous with regard to nutrient concentrations, algal biomass and dissolved metals. Specifically, the transitional zone was characterised by frequent algal blooms, resulting in fluctuating dissolved oxygen (range = 2.1-14.5 mg/ℓ) and pH (range = 7.35-10.59) levels. Using total phosphorus, Secchi depth, and chlorophyll-a concentrations, the trophic state of Loskop Dam was classified as meso-to eutrophic. Significant positive trends were observed in total (Tau = 0.422) and dissolved inorganic (Tau = 0.193) phosphorus.The reservoir showed a monomictic pattern of summer stratification (October to April) and holomictic winter circulation (June to July), with an increase in the depth and extent of anoxia in the hypolimnion when compared to previous research. Simultaneous elevated concentrations of manganese (>370 µg/ℓ) and iron in nearbottom water samples coincided with hypolimnetic anoxia. Aluminium concentrations exceeded the target water quality range (>10 µg/ℓ) during summer (December) in both surface and near-bottom water samples. We conclude that fish in Loskop Dam are periodically exposed to several physiological stressors including elevated ammonia, aluminium, iron and manganese and possibly hydrogen sulphide, as well as low dissolved oxygen. While these factors have never individually been linked to pansteatitis, their combined impacts have not been studied. To ensure the sustainability of Loskop Dam, catchment management plans must focus on reducing phosphorus inputs, and continue seeking treatment solutions for mine-water associated with abandoned and working coal mines.

Keywords:Heavy metals Oestradiol Human health Microbial pathogens Water quality abstract Water quality has deteriorated in the upper Olifants River system, South Africa, as a result of land use activities which include mining, agriculture and industries. A health risk assessment was conducted from 2009 to 2011 in the catchment to determine the possible risks local communities face from various pollutants such as microbials, heavy metals and oestrogen in the river water and vegetation. Aluminium and manganese accumulated in plants and vanadium and aluminium concentrations found in selective water samples posed significant health risks when consumed. A quantitative microbial risk assessment revealed that the combined risk of infection ranged from 1 to 26 percent with the Norovirus posing the overall greatest health risk. The anticipated disability adjusted life years resulting from drinking untreated water from these sites are in the order of 10,000 times greater than what is considered acceptable. The oestradiol activity, caused by endocrine disrupting compounds in the water, measured above the trigger value of 0.7 ng L −1 . Impoverished communities in the area, who partially depend on river water for potable and domestic use, are exposed to immune-compromising metals that increase their probability of infection from waterborne diseases caused by the excess microbial pathogens in the contaminated surface water.