A recent explosion in the amount of genomic data has revealed a large genetic diversity in the bacteriophages that infect Mycobacterium smegmatis. In an effort to assess the novelty of newly described mycobacteriophage isolates and provide a preliminary determination of their probable cluster assignment prior to full genome sequencing, we have developed a systematic approach that relies on restriction endonuclease analysis. We demonstrate that a web-based tool, the Phage Enzyme Tool (or PET), is capable of rapidly facilitating this analysis and exhibits reliability in the putative placement of mycobacteriophages into specific clusters of previously sequenced phages. We propose that this tool represents a useful analytical step in the initial study of phage genomes and that this tool will increase the efficiency of phage genome characterization and enhance the educational activities involving mycobacteriophage discovery.
This paper presents the designs for new multilayer open systems interconnect protocols that improve Internet data transmission within a hybrid communication network consisting of a geo-stationary earth orbit satellite and a terrestrial channel. These new designs span the transport, network, and data-link open systems interconnect layers and decrease the time required to transmit large data sets over a hybrid communication network compared to the Transmission Control Protocol variant Reno and frequency division multiplexing over a geo-stationary earth orbit satellite. This paper outlines the functional details for these news designs, provides simulated data transmission performance characteristics for all designs, and concludes with justifications for and benefits related to our research. 1 2
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