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The purpose of this study was to determine if calcium hydroxide, mineral trioxide aggregate, or sodium hypochlorite caused a change in the force required to fracture root dentin. Ten bovine central and lateral incisors were machined using various saws and drills to produce a cylinder of dentin with a 6.0-mm outer diameter 3.5-mm inner diameter and a length of 10 mm. The cylinders were cut lengthwise into four symmetrical pieces. The canal sides of the sections were then placed into Petri dishes containing a 1-mm depth of calcium hydroxide, mineral trioxide aggregate, sodium hypochlorite, or physiologic saline (control). The samples remained in the dishes for 5 weeks and were then shear tested by using an Instron machine. Data were analyzed using an ANOVA test for comparison of the groups as a whole, and a t test was used to compare each quarter section with its control from the same tooth. A 32% mean decrease in strength was discovered for calcium hydroxide, a 33% decrease in strength for mineral trioxide aggregate, and a 59% decrease for sodium hypochlorite. All decreases in strength were statistically significant: p < 0.001 for calcium hydroxide, p = 0.027 for mineral trioxide aggregate, and p < 0.001 for sodium hypochlorite. Results indicated that root dentin was weakened after 5 weeks of exposure to calcium hydroxide, mineral trioxide aggregate, or sodium hypochlorite.

Enterococcus faecalis tolerates highly alkaline environments, yet the exact pH required for killing E. faecalis is not known. This study tests growth at 0.5 increments from pH 9.5 to 12. Twelve culture tubes were used in each group. Positive growth was measured using turbidity, a visual scale, and a spectrophotometer. At 24 h, growth was observed in all tubes at pH 9.5 and 10. At 48 h, all pH 10.5 tubes showed growth. At 72 h, six of the pH 11 tubes showed growth. After 7 days, five of the remaining pH 11 tubes were positive. No growth occurred in any of the pH 11.5 or pH 12 tubes. Apparently, pH 10.5 to 11.0 retards growth of E. faecalis, whereas no tubes showed growth at pH 11.5 or greater.

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