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Costimulatory and coinhibitory receptor–ligand pairs on T cells and APC control the immune response. We have investigated whether selective blockade of CD28–CD80/86 costimulatory interactions, which preserves the coinhibitory CTLA4–CD80/86 interactions and the function of regulatory T (Treg) cells, abrogates the induction of experimental autoimmune encephalomyelitis (EAE) in rhesus monkeys. EAE was induced by intracutaneous immunization with recombinant human myelin oligodendrocyte glycoprotein (rhMOG) in CFA on day 0. FR104 is a monovalent, PEGylated-humanized Fab′ Ab fragment against human CD28, cross-reactive with rhesus monkey CD28. FR104 or placebo was administered on days 0, 7, 14, and 21. FR104 levels remained high until the end of the study (day 42). Placebo-treated animals all developed clinical EAE between days 12 and 27. FR104-treated animals did not develop clinical EAE and were sacrificed at the end of the study resulting in a significantly prolonged survival. FR104 treatment diminished T and B cell responses against rhMOG, significantly reduced CNS inflammation and prevented demyelination. The inflammatory profile in the cerebrospinal fluid and brain material was also strongly reduced. Recrudescence of latent virus was investigated in blood, spleen, and brain. No differences between groups were observed for the β-herpesvirus CMV and the polyomaviruses SV40 and SA12. Cross-sectional measurement of lymphocryptovirus, the rhesus monkey EBV, demonstrated elevated levels in the blood of FR104-treated animals. Blocking rhesus monkey CD28 with FR104 mitigated autoreactive T and B cell activation and prevented CNS pathology in the rhMOG/CFA EAE model in rhesus monkeys.

The measurement of moisture in building materials has been of importance to building professionals for many years to aid them in diagnosing the nature and cause of building defects. Likewise building researchers have developed an extensive selection of measurement approaches to aid them in more detailed studies of the fundamental physical processes that underlie moisture transport. This paper reviews current moisture measurement practices within the UK, and examines the more sophisticated techniques being utilised, including three particular techniques: dual probe heat pulse method; time domain reflectometry; and more sophisticated electrical approaches. These three are currently the focus of a study to evaluate how advanced research techniques can be applied to the in-situ measurement of moisture contents in practice. Practical application: This paper reviews the current UK application of measurement techniques to assessing the moisture content of building materials. It discusses the differences between the techniques used by building professionals and building researchers, and highlights potential areas currently in development which may yield more advanced site measurement options in the future.

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Synopsis Using a set-up in which the bowl of a two-point workability test apparatus was mounted on an electro-magnetic vibrating table, measurements were made on unvibrated fresh concrete and on the same samples under vibration. It was shown that when vibration is applied the flow properties of fresh concrete are no longer represented by the simple linear Bingham model but approximate to those of a power law pseudoplastic with zero yield value. At very low shear rates behaviour approximates to that of a Newtonian fluid and, subject to the exceeding of a small threshold condition, fluidity decreases as a simple exponential function of the maximum velocity of vibration.