We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells labeled with ferromagnetic nanoparticles moved upward and aligned along ferromagnetic lines deposited by lithographic techniques on an optically transparent surface of the chamber. An epi-illumination system using a 635 nm laser diode as a light source scanned the lines and measured signals obtained from the aligned cells. The cell counts per unit of blood volume obtained with the system correlated well with those obtained from the counts from a standard hematology analyzer and flow cytometer. The cell analysis platform is significantly less complex and more sensitive than current cell analysis equipment and provides additional functionality through its ability to subject the cells to repeated and varied analyses while they remain in a natural environment (i.e., whole blood).

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Serial tests for long-acting thyroid stimulator (LATS) and antithyroglobulin antibodies were performed in 32 patients with toxic diffuse goiter submitted to 13l I therapy and in 29 patients receiving antithyroid drugs. The level of detectable LATS and the incidence of positive assays tended to increase transiently following 131 I therapy and to decrease during the administration of antithyroid drugs. Statistical analysis of the LATS changes detected at the time interval of 46-180 days after treatment showed that the differences between the 2 therapeutic groups were significant. A transient rise of circulating antithyroglobulin antibodies occurred in several patients treated with radioiodine, but individual changes showed no parallelism with the course of LATS. No definite pattern was noted in the behavior of antithyroglobulin antibodies during the administration of antithyroid drugs. Twentysix patients with toxic adenoma previously treated with radioiodine and 26 untreated controls were tested for LATS and all of them had negative assays. The possibility that thiocarbamides interfere with the immune response was investigated in mice immunized with sheep red blood cells. No immunosuppressive effect of methimazole was found. The implications of these findings are discussed. (J Clin Endocr 29: 231, 1969)

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