NoteEndophytic fungi have been proved to be potent sources of novel bioactive compounds. [1][2][3] We recently reported the isolation of two new g-lactones, eutypellins A and B, two enteudesmane sesquiterpenes, ent-4(15)-eudesmen-11-ol-1-one and ent-4(15)-eudesmen-1a,11-diol, and three pimarane diterpenes, diaporthein B (3), 4) scopararane A (4), 5) and libertellenone C (5), 6) from the endophyte Eutypella sp. BCC 13199.7) Since the genus Eutypella has been rarely investigated with only a few other papers describing novel metabolites, 8,9) further chemical studies on this fungal strain was conducted. Fermentation of BCC 13199 using a bioreactor, instead of the previously employed static incubation in flasks, led to the production of two new pimarane-type diterpenes, eutypellones A (1) and B (2), together with the previously described compounds.7) In this paper we report the isolation and structure elucidation of 1 and 2, and cytotoxic activities of pimaranes 1-5.
Results and DiscussionEutypellone A (1) was isolated as a minor diterpene constituent of the broth extract. The molecular formula of 1 was determined to be C 20 H 30 O 4 by high resolution-electrospray ionization-mass spectra (HR-ESI-MS). The IR spectrum exhibited intense absorption bands at n max 3423 (broad) and 1721 cm Two new pimarane-type diterpenes, eutypellones A (1) and B (2), were isolated from the endophytic fungus Eutypella sp. BCC 13199. Cytotoxic activities of the pimaranes 1-5, isolated from this fungus, were evaluated.
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