No abstract

y Both the authors contributed equally.We compared cold static with acellular normothermic ex vivo liver perfusion (NEVLP) as a novel preservation technique in a pig model of DCD liver injury. DCD livers (60 min warm ischemia) were cold stored for 4 h, or treated with 4 h cold storage plus 8 h NEVLP. First, the livers were reperfused with diluted blood as a model of transplantation. Liver injury was determined by ALT, oxygen extraction, histology, bile content analysis and hepatic artery (HA) angiography. Second, AST levels and bile production were assessed after DCD liver transplantation. Cold stored versus NEVLP grafts had higher ALT levels (350 AE 125 vs. 55 AE 35 U/L; p < 0.0001), decreased oxygen extraction (250 AE 65 mmHg vs. 410 AE 58 mmHg, p < 0.01) and increased hepatocyte necrosis (45% vs. 10%, p ¼ 0.01). Levels of bilirubin, phospholipids and bile salts were fivefold decreased, while LDH was sixfold higher in cold stored versus NEVLP grafts. HA perfusion was decreased (twofold), and bile duct necrosis was increased (100% vs. 5%, p < 0.0001) in cold stored versus NEVLP livers. Following transplantation, mean serum AST level was higher in the cold stored versus NEVLP group (1809 AE 205 U/L vs. 524 AE 187 U/L, p < 0.05), with similar bile production (2.5 AE 1.2 cc/h vs.2.8 AE 1.4 cc/h; p ¼ 0.2). NEVLP improved HA perfusion and decreased markers of liver duct injury in DCD grafts.

Multisegment reconstruction has superior diagnostic accuracy and image quality compared with halfscan reconstruction in patients with normal heart rates.

With the advent of powerful gradient coil systems and highresolution surface coils, magnetic resonance imaging (MRI) has recently extended its role in the staging of rectal cancer. MRI is superior to endorectal ultrasound, the most widely used staging modality in patients with rectal tumors, in that it visualizes not only the intestinal wall but also the surrounding pelvic anatomy. The crucial advantage of MRI is not that it enables exact T-staging but precise evaluation of the topographic relationship of a tumor to the mesorectal fascia. This fascia is the most important anatomic landmark for the feasibility of total mesorectal excision, which has evolved into the standard operative procedure for the resection of cancer located in the middle or lower third of the rectum. MRI is currently the only imaging modality that is highly accurate in predicting whether or not it is likely that a tumorfree margin can be achieved and thus provides important information for planning of an effective therapeutic strategy, especially in patients with advanced rectal cancer.

The objective of this study was to evaluate the diagnostic yield of multislice CT using a radiation dose equivalent to that of conventional abdominal x-ray (KUB). One hundred forty-two patients were prospectively examined with ultrasound and a radically dose-reduced CT protocol (120 kV, 6.9 eff. mAs). Number and size of calculi, presence of urinary obstruction, and alternative diagnoses were recorded and confirmed by stone removal/discharge or by clinical and imaging follow-up. The mean effective whole-body dose was 0.5 mSv in men and 0.7 mSv in women. The sensitivity and specificity in detecting patients with calculi was 97% and 95% for CT and 67% and 90% for ultrasound. Urinary obstruction was similarly assessed, whereas CT identified significantly more alternative diagnoses than ultrasound (P<0.001). With regard to published data for standard-dose CT, the present CT protocol seems to be comparable in its diagnostic yield in assessing patients with calculi, and its radiation dose is equivalent to that of KUB.