BackgroundAging is associated with functional decline of neurons and increased incidence of both neurodegenerative and ocular disease. Photoreceptor neurons in Drosophila melanogaster provide a powerful model for studying the molecular changes involved in functional senescence of neurons since decreased visual behavior precedes retinal degeneration. Here, we sought to identify gene expression changes and the genomic features of differentially regulated genes in photoreceptors that contribute to visual senescence.ResultsTo identify gene expression changes that could lead to visual senescence, we characterized the aging transcriptome of Drosophila sensory neurons highly enriched for photoreceptors. We profiled the nuclear transcriptome of genetically-labeled photoreceptors over a 40 day time course and identified increased expression of genes involved in stress and DNA damage response, and decreased expression of genes required for neuronal function. We further show that combinations of promoter motifs robustly identify age-regulated genes, suggesting that transcription factors are important in driving expression changes in aging photoreceptors. However, long, highly expressed and heavily spliced genes are also more likely to be downregulated with age, indicating that other mechanisms could contribute to expression changes at these genes. Lastly, we identify that circular RNAs (circRNAs) strongly increase during aging in photoreceptors.ConclusionsOverall, we identified changes in gene expression in aging Drosophila photoreceptors that could account for visual senescence. Further, we show that genomic features predict these age-related changes, suggesting potential mechanisms that could be targeted to slow the rate of age-associated visual decline.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4304-3) contains supplementary material, which is available to authorized users.
The utilization of statistical methods an their applications within the new field of study known as Topological Data Analysis has has tremendous potential for broadening our exploration and understanding of complex, high-dimensional data spaces. This paper provides an introductory overview of the mathematical underpinnings of Topological Data Analysis, the workflow to convert samples of data to topological summary statistics, and some of the statistical methods developed for performing inference on these topological summary statistics. The intention of this non-technical overview is to motivate statisticians who are interested in learning more about the subject.
Quantitative Trait Locus (QTL) mapping of complex traits, such as leaf venation or root structures, require the phenotyping and genotyping of large populations. Sufficient genotyping is accomplished with cost effective highthroughput assays, however labor costs often makes sufficient phenotyping prohibitively limited. In order to develop efficient high-throughput phenotyping platforms for complex traits algorithms and methods for quantifying these traits are needed. It is often desirable to study the spatial organization of these phenotypes from the images generated by high-throughput platforms. With the goal of quantifying the traits, many approaches try to identify several core traits useful in describing the phenotypic morphology. This simplification may lose important information about the phenotype. Rather than reducing the structural information, we introduce a novel method, the Persistence Intensity Array, for studying complex traits using tools from the emergent field of Topological Data Analysis. This approach uses the complete geometry of the phenotype and represents it as a simpler summary of the key topological shape features contained in the data. We demonstrate this method's efficacy by through a simulated QTL analysis.
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