Background. Fatty acids (FA) likely affect human fertility at multiple levels, as deviations from physiological FA pro les are obesogenic, and FA can modify DNA methylation (DNAm). Yet, the interplay of follicular uid (FF) and serum FA with BMI and percentage body fat (PBF) in human fertility is not completely understood. Also, associations of DNAm with fertility are largely unexplored.Methods. Reproductive parameters ranging from retrieved oocyte number to infant birth weight, were recorded in Mexican women undergoing in vitro fertilization (n=88). Multiple regression analysis sought BMI-adjusted and age-adjusted associations. Receiver operating characteristic analysis tested for discrimination between outcomes.Results. Associations of FF and serum FA were markedly distinct. While various FF FA (C16:1, C18:0, C20:2, C20:3, arachidonic acid) were signi cantly and inversely associated only with retrieved oocyte number, selected serum FA were associated with a broad range of pre-fertilization and post-fertilization parameters. Associations of BMI and FF FA were complex, as arachidonic acid was inversely associated with both BMI and retrieved oocyte number, while oleic acid (OA) was directly associated with BMI and PBF. Ultrasound-assessed clinical pregnancy outcome (CP) was directly associated with serum OA but inversely with its trans isomer elaidic acid (EA) and with BMI. Compounded BMI, serum EA and OA discriminated CP well (AUC=0.74). Whole blood DNA methylation was signi cantly associated with and a moderate predictor (AUC=0.66) of percent fertilized oocytes.Conclusions. Overall FF FA pool composition rather than FA identity may impact oocyte production and cellular memory of FF FA is lost as the oocyte exits the follicular environment. The contrasting associations of BMI, FF OA and arachidonic acid suggest that the control of oocyte homeostasis by FF FA is uncoupled from BMI. Further studies are warranted to assess the potential of compounding BMI with serum EA and OA to predict CP.
and the use of ovary and oocyte cryopreservation techniques based on human procedures. In conclusion, we report the successful cryopreservation of Mexican gray wolf gametes based only in local resources and expertise. Our results will impact the long-standing efforts of the population management program to recover a flagship species of Mexican natural richness, the gray wolf.
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