Recovery studies were done with bee pollen spiked with eight organochlorine pesticides. Matrix solidphase dispersion was used to extract pesticides from bee pollen using octadecyl silica (C18, 0.5 g) as solid support. The co-column material for clean-up (graphitized carbon and primary secondary amine) and the eluting system (acetonitrile or acetonitrile/ethyl acetate) were optimized. Pesticide recoveries were determined by GC-MS-SIM through external calibration curves. The recoveries obtained without co-column and primary secondary amine (PSA) did not differ significantly and were higher than those obtained using graphitized carbon. With PSA used as the co-column, chromatograms with low background, low intensity, and fewer peaks were acquired. Thus, primary secondary amine was used to clean up the pollen extracts. Recoveries were higher than 100 % and precisions were poor for most of the pesticides when acetonitrile/ethyl acetate was used to elute them. Therefore, acetonitrile was selected for the elution of organochlorine pesticides. The final extraction conditions were: C18 (adsorbent), primary secondary amine (co-column), and ACN (eluting solvent). Recoveries obtained ranged from 41 to 101 %.
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