Zebrafish (Danio rerio) is an ideal in vivo model to study a wide variety of human cancer types. In this review, we provide a comprehensive overview of zebrafish in the cancer drug discovery process, from (i) approaches to induce malignant tumors, (ii) techniques to monitor cancer progression, and (iii) strategies for compound administration to (iv) a compilation of the 355 existing case studies showing the impact of zebrafish models on cancer drug discovery, which cover a broad scope of scenarios. Finally, based on the current state-of-the-art analysis, this review presents some highlights about future directions using zebrafish in cancer drug discovery and the potential of this model as a prognostic tool in prospective clinical studies.

<p>This file contains supplemental tables of advantages and disadvantages of methods developed to induce cancer in zebrafish and the advantages and disadvantages of using embryo or adult stage in research assays</p>

<p>This file contains supplemental tables of zebrafish cancer models induced by chemical mutagenesis and by gene editing techniques</p>

<p>This file contains supplemental tables of zebrafish cancer models carried out in zebrafish using genetics approaches, allograft, xenograft and orthotopic assays</p>

<p>This file contains imaging techniques to monitor tumor process in zebrafish cancer models</p>

<p>This file contains 355 reported case studies in zebrafish in cancer drug discovery projects</p>

<p>This file contains supplemental tables of zebrafish cancer models induced by chemical mutagenesis and by gene editing techniques</p>

<p>This file contains 355 reported case studies in zebrafish in cancer drug discovery projects</p>