Este estudo retrata a fauna de equinodermos encontrada na Praia do Cabo Branco, João Pessoa, Paraíba, Brasil. Esta praia é formada por um terraço de abrasão marinha do tipo arenito-ferruginoso e está localizada no ponto mais oriental das Américas. Foram identificadas 32 espécies de equinodermos pertencentes a quatro classes (Asteroidea, Ophiuroidea, Echinoidea e Holothuroidea), distribuídas em 18 famílias e 24 gêneros. O número de táxons identificados corresponde a 9,7% das espécies registradas para o litoral Brasileiro e mostra a significativa diversidade das espécies de Echinodermata encontrada nesta área. A classe Ophiuroidea foi a mais diversa com 13 espécies, seguida por Holothuroidea (10 spp.), Asteroidea (5 spp.) e Echinoidea (4 spp). Do total de espécies registradas, as seguintes são novas ocorrências para o litoral do Estado da Paraíba: Ophiocnida scabriuscula, Ophiomyxa flaccida, Ophiactis lymani, Ophionereis reticulata, Ophioderma januarii, Ophiophragmus brachyatis, Thyone cognita, T. suspecta, Ocnus suspectus, Phyllophorus occidentalis, P. communis, Lissothuria sp. Holothuria grisea, H. arenicola, Chiridota rotifera, Synaptula hydriformis, Echinaster nudus, Linckia guildingii, Lytechinus variegatus, Mellita quinquiesperforata e Leodia sexiesperforata.
ABSTRACT. Ucides cordatus (Linnaeus, 1763) is a hypo-hyper-regulating mangrove crab possessing gills for respiratory and osmoregulatory processes, separated in anterior and posterior sections. They also have hepatopancreas, which is responsible for digestion and absorption of nutrients and detoxification of toxic metals. Each of these organs has specific cells that are important for in vitro studies in cell biology, ion and toxic metals transport. In order to study and characterize cells from gills and hepatopancreas, both were separated using a Sucrose Gradient (SG) from 10 to 40% and cells in each gradient were characterized using the vital mitochondrial dye DASPEI (2-(4-dimethylaminostyryl)-N-ethylpyridinium iodide) and Trichrome Mallory's stain. Both in 20 and 40% SG for gill cells and 30% SG for hepatopancreatic cells, a greater number of cells were colored with DASPEI, indicating a larger number of mitochondria in these cells. It is concluded that the gill cells present in 20% and 40% SG are Thin cells, responsible for respiratory processes and Ionocytes responsible for ion transport, respectively. For hepatopancreatic cells, the 30% SG is composed of Fibrillar cells that possess larger number of membrane ion and nutrient transporters. Moreover, the transport of toxic metal cadmium (Cd) by isolated hepatopancreatic cells was performed as a way of following cell physiological integrity after cell separation and to study differences in transport among the cells. All hepatopancreatic cells were able to transport Cd. These findings are the first step for further work on isolated cells of these important exchange epithelia of crabs, using a simple separation method and to further develop successful in vitro cell culture in crabs. KEYWORDS.Mangrove crab, DASPEI, cell cadmium transport, in vitro cells. RESUMO. Caracterização citoquímica de células de brânquias e hepatopâncreas deUcides cordatus (Crustacea, Brachyura) validado por transporte celular de metais. Ucides cordatus (Linnaeus, 1763) é um caranguejo de mangue hiper-hipo-regulador que possui brânquias responsáveis por processos respiratórios e osmorregulatórios. Apresentam também hepatopâncreas, que realizam digestão, absorção de nutrientes e detoxificação de metais tóxicos. Cada um desses órgãos possui células específicas que são importantes para estudos in vitro em biologia celular e transporte de íons e metais tóxicos. Visando estudar e caracterizar as células de cada órgão, estas foram separadas em um gradiente de sacarose (SG) de 10 a 40% e as células de cada gradiente foram caracterizadas usando o marcador de mitocôndria DASPEI (2-(4-dimethylaminostyryl)-N-ethylpyridinium iodide) e o corante Tricromo de Mallory. No SG de 20 e 40% para células branquiais e 30% para células hepatopancreáticas, foi observado um maior número de células coradas com DASPEI, indicando um maior número de mitocôndrias nessas células. Conclui-se que células branquiais presentes no SG de 20 e 40% são "Thin cells", responsáveis por processos respiratórios, e Ionócitos, responsáv...
Rhodolith beds formed by non-articulated calcareous algae have been recognized as important habitats for a range of organisms. The Brazilian coast is home to one of the largest beds of the Southwestern Atlantic, but the lack of faunal studies in these habitats represents one of our major gaps in the knowledge of Brazilian biodiversity. This study compares the composition, abundance and diversity of echinoderm species associated with rhodoliths in three different isobaths (10, 15 and 20 m) along the coast of the State of Paraíba, Northeastern Brazil. These rhodolith beds provided 2,855 specimens, representing 32 species from four Classes, of which Ophiuroidea was the most numerous. The echinoderm community was significantly different considering the abundance of individuals (F=452.86, p=0.001) and the number of species (F=45.14, p=0.006) among the three depths analyzed. Abundance and species numbers decrease with increasing depth. Rhodolith beds in the State of Paraíba are an important habitat for echinoderms, harboring a high diversity and abundance of associated species. Richness and diversity records exceed those found in other coastal areas around the world, indicating that these banks are of special relevance for the conservation of echinoderms in Brazil.
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