Valuable female cattle are continuously subject to follicular puncture (ovum pickup -opU). this technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative effects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5 × 10 6 MSCs immediately after the 4th OPU procedure (n = 5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P < 0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC + FSH in both ovaries after the first OPU. In the two following OPU sessions, the MSC and MSc + FSH-treated groups failed to present any significant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have beneficial effects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.

Objective: To evaluate the use of mesenchymal stem cells (MSCs) in the attenuation of canine atopic dermatitis (AD). Materials and methods: Sixteen dogs were selected and divided into three groups, mild, moderate, and severe, according to the Canine Atopic Dermatitis Extent and Severity Index (CADESI-4). They were evaluated for 82 days. The protocol recommended in this experiment was to inject 2 × 10 6 /kg bodyweight of MSC’s in all groups by the intravenous route with intervals of applications of 21 days. The degree of pruritus was evaluated by examining the visual analog scale, the CADESI-4, the histopathology of the skin, hematological and biochemical parameters, the pyogenic effect of MSCs, and the thickness of the epidermis. Results: There was a significant difference in the reduction of epidermal thickness in the moderate and severe groups. Hematological, biochemical, and body temperature parameters remained within normal limits for the species with no side effects Conclusion: MSCs attenuated the clinical signs of AD.

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A doença renal crônica (DRC), enfermidade caracterizada pela inflamação túbulo-intersticial, atrofia tubular e fi-brose intersticial com perda progressiva da função renal, tem se tornado uma afecção relativamente comum em cães e gatos. Devido ao fato de o transplante renal ainda não ser uma opção viável na medicina veterinária, surgi-ram estudos para avaliar a possibilidade de tratamento de DRC com células-tronco mesenquimais (CTMs), em ra-zão dos efeitos anti-inflamatórios, anti-oxidantes, anti-fibróticos e imunomoduladores que essas células possuem. Onze pacientes portadores de DRC (6 cães e 5 gatos) foram avaliados e submetidos à terapia com CTMs halógenas. Todos os 5 gatos e 4 dos 6 cães tiveram diminuição nas taxas de creatinina sérica após o transplante de células, indicando que houve diminuição significativa para a creatinina em cães (p=0.0238). Houve, também, considerável diminuição na frequência de vômitos (p<0.05) e aumento no apetite (p<0.05) dos animais que receberam a terapia com células, de acordo com a percepção dos tutores. Esses resultados sugerem que a terapia com CTMs pode ser avaliada como uma alternativa para os animais portadores de DRC. Novos estudos devem ser realizados, com acompanhamento por maior espaço de tempo afim de estabelecer a melhor concentração de células e o número ideal de aplicações para estabilização dos pacientes portadores de DRC.

BackgroundThe cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR.MethodsThe MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups. Animals from the treated group (GT) received cell transport medium containing about 1.5 millions MSC, and the animals from the control group (GC) received only the cell transport medium. The study was performed in a double-blind manner using radiographs acquired on days 15, 30, 60 and 120 after the procedure. Evaluations of the density of the trabecular bone were performed using image analysis software. The results were subjected to descriptive statistical analysis, followed by the normality test, Chi-square test, Mann-Whitney test and Tukey’s multiple comparison test for p ≤ 0.05.ResultsAfter 30 days of the procedure, the animals of the GT presented an ossification mean 36.45% greater (p ≤ 0.033) than the GC, and there were no statistical differences for the other periods.ConclusionsDespite the total bone ossification within the expected period, there was no minimization of the estimated recovery time with the application of MSC, and inflammatory factors should be considered for reassessment of the therapeutic intervention time.