RESUMO -Explantes de bananeira cv. Caipiras (AAA) foram cultivados in vitro. Nas subculturas de estabelecimento e multiplicação, o meio utilizado foi MS + 5 mg/l de BAP e, na de enraizamento, 50%MS sem BAP. Na avaliação das subculturas, a maior contaminação ocorreu no estabelecimento (74,7%), seguindo decrescente até o enraizamento, com 5,6%. O número de brotos por frasco diminui ao longo das subculturas, atingindo 3,7 brotos na subculturas 1 e 1,7 na subcultura 4. O tamanho dos brotos permaneceu entre 24 e 26 mm, nas subculturas de 1 a 3, diminuindo na subcultura 4, com 22 mm. Como conseqüência, a classe de brotos menores (x ≤ 20 mm) predominou, variando de 56% na subcultura 1 para 65% na subcultura 4. No enraizamento, a predominância (62%) foi de brotos na faixa entre 30 e 60 mm, com média de 4,6 brotos por frasco. A avaliação geral do processo indicou um rendimento real de 70,9 mudas/rizoma. Palavras-chave: Micropropagação, Banana, Cultura de Tecidos EVALUATION OF A COMMERCIAL PROTOCOL FOR IN VITRO MULTIPLICATION OF BANANA (Musa sp) CV. CAIPIRA (AAA) ABSTRACT:Banana explants cv. Caipira (AAA) was cultivated in vitro. In the establishment and multiplication phases the medium used was MS+5mg/l BAP and in the rooting phase, 50%MS without BAP. In the subcultures evaluations, the highest contamination rate occurred at the establishment (74,7%), decreasing until rooting, with 5,6%. The number of shoots per flask along the subcultures was 3,7 in the first subculture and 1,7 in the fourth. The shoots' size remained between 24 and 26 mm in the subcultures 1 to 3, reducing in the subculture 4 with 22 mm. As a consequence, the class of smaller shoots (x ≤ 20 mm) was predominant, from 56% in the subculture 1 to 65% in the subculture 4. During rooting, the predominance (62%) of shoots was between 30 and 60 mm, with 4,6 shoots per flask. The general evaluation of the process indicated an efficiency of 70,9 plantlets per rhizome.
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