The space and time resolutions used for the input variables of a distributed hydrological model have a sufficient impact on the model results. This resolution depends on the required accuracy, experimental site and the processes and variables taken into account in the hydrological model. The influence of space and time resolution is studied here for the case of TOPMODEL, a model based on the variable contributing area concept, applied to an experimental 12 km2 catchment (Coet-Dan, Brittany, France) during a two month winter period. A sensitivity analysis to space and time resolution is performed first for input variables derived from the digital elevation data, secondly for the optimized values of the TOPMODEL parameters and finally for modelling efficiency. This analysis clearly shows that a relevant domain of space and time resolutions where efficiency is fairly constant can be defined for the input topographic variables, as opposed to another domain of larger resolutions that induces a strong decrease of modelling effciency. It also shows that the use of a single set of parameters, defined as mean values of parameters on this relevant domain of resolution, does not modify the accuracy of modelling. The sensitivity of the parameters to space and time resolution allows the physical significance of the parameter values to be discussed.

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The insulin-like peptide human relaxin-2 was identified as a hormone that, among other biological functions, mediates the hemodynamic changes occurring during pregnancy. Recombinant relaxin-2 (serelaxin) has shown beneficial effects in acute heart failure, but its full therapeutic potential has been hampered by its short halflife and the need for intravenous administration limiting its use to intensive care units. In this study, we report the development of long-acting potent single-chain relaxin peptide mimetics. Modifications in the B-chain of relaxin, such as the introduction of specific mutations and the trimming of the sequence to an optimal size, resulted in potent, structurally simplified peptide agonists of the relaxin receptor Relaxin Family Peptide Receptor 1 (RXFP1) (e.g., 54). Introduction of suitable spacers and fatty acids led to the identification of single-chain lipidated peptide agonists of RXFP1, with subnanomolar activity, high subcutaneous bioavailability, extended half-lives, and in vivo efficacy (e.g., 64).

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In grassland ecosystems, soil animals act as key soil engineers and architects. The diversity of soil animals is also a regulator of ecosystem carbon flow. However, our understanding of the link between soil animals, carbon fluxes and soil physical organisation remains poor. An integrated approach based on soil micromorphology and laser ablation stable isotope ratio mass spectrometry (LA-IRMS) was developed to provide spatially distributed data of pulse-derived (13)C tracer from roots in the soil environment. This paper describes the development and testing of a LA-IRMS (13)C/(12)C analytical method on soil thin sections as a means to determine the fate of root carbon derived from photosynthesis into soil. Results from this work demonstrated (1) that micro-scale delta(13)C (per thousand) analysis could be made on targeted features located within a soil thin section and (2) that LA-IRMS delta(13)C (per thousand) measurements made on samples obtained from (13)CO(2) pulse labelled plant-soil blocks confirmed the presence of recent photosynthates in the rhizosphere (1 and 4 weeks post-pulse).

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