In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.
In this work, a mesoporous c-Al 2 O 3 membrane was synthesised on an a-Al 2 O 3 substrate by a solgel dip coating process. The membranes were characterised using SEM, field emission SEM, Xray diffractometry and N 2 adsorption/desorption measurements (Brunauer-Emmett-Teller analyses). The characterisation results revealed that a flawless c-alumina membrane layer with 3?5 mm thickness and 2?1 nm average pore size was achieved. Subsequent separation tests indicated that CO 2 could be separated from N 2 by the mesoporous c-Al 2 O 3 membrane. In spite of the difficulties of the separation, the optimised microstructure achieved for the prepared membrane led to desirable and regular permeation behaviour. It was also observed that separation could be more efficient in high pressure permeation conditions. The prepared substrate fulfilled the required strength and permeability for the thin c-Al 2 O 3 membrane film under these conditions. Accordingly, an optimised completely ceramic membrane structure, applicable for CO 2 separation applications, was achieved.
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