nated eggs immediately after the interfering virus. In contrast, 128 agglutinating doses of virus inactivated with 10 times less mustard at pH 6.2 caused complete suppression of 3 16 ID30 and partial suppression of 3 160 ID50 of challenge virus. In fact, even 64 agglutinating doses of this interfering preparation afforded marked inhibition of multiplication of 3 16 of active virus. A prevailing concept of mustard action assumes an activation of mustard molecules in polar solvents and intramolecular transformations leading to production of unstable and reactive cyclic intermediates. Since the formation of these cyclic intermediates is repressed by acidic reactions, the present investigations were initiated to study the modifying influence of pH upon mustard inactivation of viral infectivity. Suntinmy. A more complete destruction of virus occurred at acid reactions (pH 6.2) as opposed to alkaline reactions. The results with 5 x lo-'] 11 mustard also indicated that at alkaline reactions destruction of viral in-Discussion. TUBERCLE BACILLIfectivity was completed during the first half minute of mustard-virus interaction. In contrast, at pH 6.2 the action of mustard upon virus extended beyond this half minute and led to complete inactivation of virus which survived the initial period of reaction. 2. A comparison of the interfering capacity of virus inactivated with 5 x 31 mustard at p H 8.0 with that of virus treated with 5 x M mustard at pH 6.2 revealed a marked interfering effect for the latter. This observation would appear to suggest a convenient procedure for preparation of interfering suspensions of virus for studies of the interference phenomenon.
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