The G-quadruplexes that form in the
HIV-1 RNA genome hinder progression
of reverse transcriptase
in vitro, but not in infected cells. We investigated the possibility
that the HIV-1 nucleocapsid protein NCp7, which remains associated
with the viral RNA during reverse transcription, modulated HIV-1 RNA
G-quadruplex stability. By electrophoresis, circular dichroism, mass
spectrometry, and reverse transcriptase stop assays, we demonstrated
that NCp7 binds and unfolds the HIV-1 RNA G-quadruplexes and promotes
DNA/RNA duplex formation, allowing reverse transcription to proceed.
The G-quadruplex ligand BRACO-19 was able to partially counteract
this effect. These results indicate NCp7 as the first known viral
protein able to unfold RNA G-quadruplexes, and they explain how the
extra-stable HIV-1 RNA G-quadruplexes are processed; they also point
out that the reverse transcription process is hindered by G-quadruplex
ligands at both reverse transcriptase and NCp7 level. This information
can lead to the development of more effective anti-HIV-1 drugs with
a new mechanism of action.
G-quadruplexes (G4s) are four-stranded nucleic acid structures abundant at gene promoters. They can adopt several distinctive conformations. G4s have been shown to form in the herpes simplex virus-1 (HSV-1) genome during its viral cycle. Here by cross-linking/pull-down assay we identified ICP4, the major HSV-1 transcription factor, as the protein that most efficiently interacts with viral G4s during infection. ICP4 specific and direct binding and unfolding of parallel G4s, including those present in HSV-1 immediate early gene promoters, induced transcription in vitro and in infected cells. This mechanism was also exploited by ICP4 to promote its own transcription. Proximity ligation assay allowed visualization of G4-protein interaction at the single selected G4 in cells. G4 ligands inhibited ICP4 binding to G4s. Our results indicate the existence of a well-defined G4-viral protein network that regulates the productive HSV-1 cycle. They also point to G4s as elements that recruit transcription factors to activate transcription in cells.
G-quadruplexes (G4s), four-stranded nucleic acid structures that adopt several distinctive conformations, are abundant at gene promoters and have been proposed as transcription regulatory elements. G4s form in the herpes simplex virus-1 (HSV-1) genome during its viral cycle. Here by cross-linking/pull-down assay we identified ICP4 as the protein that most interacts with viral G4s during infection. In vitro and in infected cells, ICP4 specifically and directly bound and unfolded parallel G4s, including those present in HSV-1 immediate early gene promoters, and consequently induced transcription. This mechanism was also exploited by ICP4 to promote its own transcription. By proximity ligation assay we visualized ICP4 interaction at the single G4 in cells. G4 ligands inhibited ICP4 binding to G4s. Our results indicate the existence of a well-defined G4-viral protein network that regulates the productive HSV-1 cycle. They also point to G4s as elements that recruit transcription factors to activate transcription in cells.
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