The effect of soil moisture on bacterial growth was investigated, and the effects of rewetting were compared with glucose addition because both treatments increase substrate availability. Bacterial growth was estimated as thymidine and leucine incorporation, and was compared with respiration. Low growth rates were found in air-dried soil, increasing rapidly to high stable values in moist soils. Respiration and bacterial growth at different soil moisture contents were correlated. Rewetting air-dried soil resulted in a linear increase in bacterial growth with time, reaching the levels in moist soil (10 times higher) after about 7 h. Respiration rates increased within 1 h to a level >10 times higher than that in moist soil. After the initial flush, there was a gradual decrease in respiration rate, while bacterial growth increased to levels twice that of moist soil 24 h after rewetting, and decreased to levels similar to those in moist soil after 2 days. Adding glucose resulted in no positive effect on bacterial growth during the first 9 h, despite resulting in more than five times higher respiration. This indicated that the initial increase in bacterial growth after rewetting was not due to increased substrate availability.

The effects of municipal food waste compost addition and mineral fertilization on selected soil microbiological activities were investigated during 3 years of reiterated treatments on two Mediterranean agricultural soils with different organic carbon content. Compost at 15, 30 and 45 t ha−1 (dry matter), mineral (NPK) fertilizers and combined fertilizers with 15 t ha−1 of compost plus two reduced doses of mineral N were applied to both soils. At both sites, organic amendment increased soil respiration, fluorescein diacetate hydrolysis, phosphatase and arylsulphatase activities. The differences in soil microbial activities\ud among treatments, found after 3 years of repeated treatments, were attributable to the variations of soil organic C content and to the impact of soil tillage. Our results show that, in Mediterranean intensively cultivated agroecosystems, annual organic amendments improve the microbial activity of soil and produce cumulative effects, suggesting the usefulness of repeated high-rate compost applications

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The maintenance of certain levels of old forest represents a cornerstone of the EU's biodiversity management strategy. A consensus on a single general ecological definition of old-growth is particularly difficult in Mediterranean Europe. The present paper deals with old-growth forests and persistent woodlands in the Cilento and Vallo di Diano National Park (PNCVD) to give an ecological understanding of forest complexity and dynamics under a multiscale and multidisciplinary perspective. The multiscale approach ranged from the identification and mapping of potential old-growth stands at landscape scale to a two-level field review of forest stand features. Field sampling involved a multidisciplinary team of researchers in forest structure, pedologic environment, soil microbial activity, flora and vegetation and deadwood components. The research provided sound knowledge about old-growthness features in the PNCVD that constitutes a unique case study in the whole Mediterranean basin. The integration of results allowed to: identify main ecosystem functions and the related services of the old-growth forests in the study area; distinguish persistent woodlands, multi-aged stands with old trees deriving from nineteenth-century management practices, from old-growth forests sensu strictu ; recognize indicators of direct and indirect impacts of human activities; suggest effective practices for sustainable management in the Mediterranean context.