Information entropy is used to summarize transcriptome data, but ignoring zero count data contained them. Ignoring zero count data causes loss of information and sometimes it was difficult to distinguish between multiple transcriptomes. Here, we estimate Kolmogorov complexity of transcriptome treating zero count data and distinguish similar transcriptome data.

Mahafacyclin B is a cyclic peptide isolated from the latex of Jatropha mahafalensis and is an antimalarial agent. However, the physiological effects of mahafacyclin B in mammalian cells are not known. Here, we assessed the growth, morphology, and alterations in the transcriptome of CHO-K1 cells exposed to mahafacyclin B (0–22 μM). Mahafacyclin B at 2.2 μM did not affect the proliferation or death of CHO-K1 cells. Mahafacyclin B was not toxic to mammalian cells at 2.2 μM, which represents a normal physiological concentration at which mahafacyclin B retains its antimalarial properties. Interestingly, mahafacyclin B altered the size and morphology of CHO-K1 cells. Comparative transcriptomics revealed that mahafacyclin B modulated the expression of a specific subset of genes.

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