The interaction between gut microbiota and host immunity shapes the immune development and microbial functions. Dysbiosis is known to relate to allergy. However, whether alteration of gut microbiota is different among distinct allergic phenotypes has never been elucidated. Our study aimed to determine the difference between gut microbiota and microbial products among infants with different allergic phenotypes from a longitudinal birth cohort in Bangkok, Thailand. METHODS: Gut microbiome of 26 allergic infants and 26 matchedhealthy controls at aged 9-12 months were analyzed using 16s amplicon sequencing. Proteomics analysis was performed using LC-MS. RESULTS: Subjects with atopic manifestation were grouped into 3 phenotypes; atopic dermatitis(AD)61.6%, food allergy(FA)19.2% and subjects with both AD and FA(AD/FA)19.2%. While subjects with only AD had normal microbial diversity, ones with FA and AD/FA showed lowdiversity microbial communities. Erysipelotrichaceae was the most significant abundance in AD. It expressed proteins related to cell replication such as DNA polymerase-I, suggesting its high activity. Bifidobacteriaceae was less abundance in allergic populations and also showed decreased production of glycosyltransferase, which stimulates intestinal mucus synthesis and decreased proteins involving in bacterial cellular energy production/ nutrition uptake such as ATP synthase. Altogether suggested the inactivity and malfunction of Bifidobacteriaceae in allergic subjects. CONCLUSIONS: Different microbial patterns were demonstrated in subjects with distinct allergic phenotypes. Less diversity was found in FA while specific strains of microbiota predominated in AD. Understanding the dynamics of microbial colonization patterns and their activities provides insight into the pathophysiology of allergic diseases as well as the potential target for allergy prevention and treatment.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.